Detection of c-Myc protein-protein interactions and phosphorylation status by immunoprecipitation

Colin J. Daniel, Xiaoli Zhang, Rosalie C. Sears

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Co-immunoprecipitation is an invaluable technique in evaluating native protein-protein interactions in vitro and in vivo. However, it can be difficult to detect interactions of a very transient nature, particularly interactions with phosphatases and kinases. The evaluation of the phosphorylation status of c-Myc can also be challenging with the current commercially available phosphorylation sensitive antibodies. Here, we describe two protocols: one for the co-immunoprecipitation of endogenous c-Myc to detect protein-protein interactions and second, for the immunoprecipitation of endogenous c-Myc to probe for phosphorylation status.

Original languageEnglish (US)
Title of host publicationThe Myc Gene
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages65-76
Number of pages12
ISBN (Print)9781627034289
DOIs
StatePublished - Jan 1 2013

Publication series

NameMethods in Molecular Biology
Volume1012
ISSN (Print)1064-3745

Keywords

  • Axin1
  • B56α
  • GSK3β
  • HBP1
  • Immunoprecipitation
  • PP2A
  • Phosphorylation
  • c-Myc

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Daniel, C. J., Zhang, X., & Sears, R. C. (2013). Detection of c-Myc protein-protein interactions and phosphorylation status by immunoprecipitation. In The Myc Gene: Methods and Protocols (pp. 65-76). (Methods in Molecular Biology; Vol. 1012). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-429-6_5