Detection of c-Myc protein-protein interactions and phosphorylation status by immunoprecipitation

Colin J. Daniel, Xiaoli Zhang, Rosalie Sears

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Co-immunoprecipitation is an invaluable technique in evaluating native protein-protein interactions in vitro and in vivo. However, it can be difficult to detect interactions of a very transient nature, particularly interactions with phosphatases and kinases. The evaluation of the phosphorylation status of c-Myc can also be challenging with the current commercially available phosphorylation sensitive antibodies. Here, we describe two protocols: one for the co-immunoprecipitation of endogenous c-Myc to detect protein-protein interactions and second, for the immunoprecipitation of endogenous c-Myc to probe for phosphorylation status.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages65-76
Number of pages12
Volume1012
ISBN (Print)9781627034289
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1012
ISSN (Print)10643745

Fingerprint

Proto-Oncogene Proteins c-myc
Immunoprecipitation
Phosphorylation
Proteins
Phosphoric Monoester Hydrolases
Phosphotransferases
Antibodies

Keywords

  • Axin1
  • B56α
  • c-Myc
  • GSK3β
  • HBP1
  • Immunoprecipitation
  • Phosphorylation
  • PP2A

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Daniel, C. J., Zhang, X., & Sears, R. (2013). Detection of c-Myc protein-protein interactions and phosphorylation status by immunoprecipitation. In Methods in Molecular Biology (Vol. 1012, pp. 65-76). (Methods in Molecular Biology; Vol. 1012). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-429-6_5

Detection of c-Myc protein-protein interactions and phosphorylation status by immunoprecipitation. / Daniel, Colin J.; Zhang, Xiaoli; Sears, Rosalie.

Methods in Molecular Biology. Vol. 1012 Humana Press Inc., 2013. p. 65-76 (Methods in Molecular Biology; Vol. 1012).

Research output: Chapter in Book/Report/Conference proceedingChapter

Daniel, CJ, Zhang, X & Sears, R 2013, Detection of c-Myc protein-protein interactions and phosphorylation status by immunoprecipitation. in Methods in Molecular Biology. vol. 1012, Methods in Molecular Biology, vol. 1012, Humana Press Inc., pp. 65-76. https://doi.org/10.1007/978-1-62703-429-6_5
Daniel CJ, Zhang X, Sears R. Detection of c-Myc protein-protein interactions and phosphorylation status by immunoprecipitation. In Methods in Molecular Biology. Vol. 1012. Humana Press Inc. 2013. p. 65-76. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-429-6_5
Daniel, Colin J. ; Zhang, Xiaoli ; Sears, Rosalie. / Detection of c-Myc protein-protein interactions and phosphorylation status by immunoprecipitation. Methods in Molecular Biology. Vol. 1012 Humana Press Inc., 2013. pp. 65-76 (Methods in Molecular Biology).
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