@inbook{f9e010f72f8041f1abf6948ef84c4012,
title = "Detection of c-Myc protein-protein interactions and phosphorylation status by immunoprecipitation",
abstract = "Co-immunoprecipitation is an invaluable technique in evaluating native protein-protein interactions in vitro and in vivo. However, it can be difficult to detect interactions of a very transient nature, particularly interactions with phosphatases and kinases. The evaluation of the phosphorylation status of c-Myc can also be challenging with the current commercially available phosphorylation sensitive antibodies. Here, we describe two protocols: one for the co-immunoprecipitation of endogenous c-Myc to detect protein-protein interactions and second, for the immunoprecipitation of endogenous c-Myc to probe for phosphorylation status.",
keywords = "Axin1, B56α, GSK3β, HBP1, Immunoprecipitation, PP2A, Phosphorylation, c-Myc",
author = "Daniel, {Colin J.} and Xiaoli Zhang and Sears, {Rosalie C.}",
year = "2013",
doi = "10.1007/978-1-62703-429-6_5",
language = "English (US)",
isbn = "9781627034289",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "65--76",
booktitle = "The Myc Gene",
}