Detection and mapping of amplified DNA sequences in breast cancer by comparative genomic hybridization

Anne Kallioniemi; Olli Pekka Kallioniemi; Jim Piper; Minna Tanner; Trond Stokke; Ling Chen; Helene S. Smith; Dan Pinkel; Joe W. Gray; Frederic M. Waldman

doi:10.1073/pnas.91.6.2156

Detection and mapping of amplified DNA sequences in breast cancer by comparative genomic hybridization

Anne Kallioniemi, Olli Pekka Kallioniemi, Jim Piper, Minna Tanner, Trond Stokke, Ling Chen, Helene S. Smith, Dan Pinkel, Joe W. Gray, Frederic M. Waldman

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Abstract

Comparative genomic hybridization was applied to 5 breast cancer cell lines and 33 primary tumors to discover and map regions of the genome with increased DNA-sequence copy-number. Two-thirds of primary tumors and almost all cell lines showed increased DNA-sequence copy-number affecting a total of 26 chromosomal subregions. Most of these loci were distinct from those of currently known amplified genes in breast cancer, with sequences originating from 17q22-q24 and 20q13 showing the highest frequency of amplification. The results indicate that these chromosomal regions may contain previously unknown genes whose increased expression contributes to breast cancer progression. Chromosomal regions with increased copy-number often spanned tens of Mb, suggesting involvement of more than one gene in each region.

Original language	English (US)
Pages (from-to)	2156-2160
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	91
Issue number	6
DOIs	https://doi.org/10.1073/pnas.91.6.2156
State	Published - Mar 15 1994
Externally published	Yes

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Kallioniemi, A., Kallioniemi, O. P., Piper, J., Tanner, M., Stokke, T., Chen, L., Smith, H. S., Pinkel, D., Gray, J. W., & Waldman, F. M. (1994). Detection and mapping of amplified DNA sequences in breast cancer by comparative genomic hybridization. Proceedings of the National Academy of Sciences of the United States of America, 91(6), 2156-2160. https://doi.org/10.1073/pnas.91.6.2156

Kallioniemi, A, Kallioniemi, OP, Piper, J, Tanner, M, Stokke, T, Chen, L, Smith, HS, Pinkel, D, Gray, JW & Waldman, FM 1994, 'Detection and mapping of amplified DNA sequences in breast cancer by comparative genomic hybridization', Proceedings of the National Academy of Sciences of the United States of America, vol. 91, no. 6, pp. 2156-2160. https://doi.org/10.1073/pnas.91.6.2156

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abstract = "Comparative genomic hybridization was applied to 5 breast cancer cell lines and 33 primary tumors to discover and map regions of the genome with increased DNA-sequence copy-number. Two-thirds of primary tumors and almost all cell lines showed increased DNA-sequence copy-number affecting a total of 26 chromosomal subregions. Most of these loci were distinct from those of currently known amplified genes in breast cancer, with sequences originating from 17q22-q24 and 20q13 showing the highest frequency of amplification. The results indicate that these chromosomal regions may contain previously unknown genes whose increased expression contributes to breast cancer progression. Chromosomal regions with increased copy-number often spanned tens of Mb, suggesting involvement of more than one gene in each region.",

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AU - Kallioniemi, Anne

AU - Kallioniemi, Olli Pekka

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AU - Stokke, Trond

AU - Chen, Ling

AU - Smith, Helene S.

AU - Pinkel, Dan

AU - Gray, Joe W.

AU - Waldman, Frederic M.

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AB - Comparative genomic hybridization was applied to 5 breast cancer cell lines and 33 primary tumors to discover and map regions of the genome with increased DNA-sequence copy-number. Two-thirds of primary tumors and almost all cell lines showed increased DNA-sequence copy-number affecting a total of 26 chromosomal subregions. Most of these loci were distinct from those of currently known amplified genes in breast cancer, with sequences originating from 17q22-q24 and 20q13 showing the highest frequency of amplification. The results indicate that these chromosomal regions may contain previously unknown genes whose increased expression contributes to breast cancer progression. Chromosomal regions with increased copy-number often spanned tens of Mb, suggesting involvement of more than one gene in each region.

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Detection and mapping of amplified DNA sequences in breast cancer by comparative genomic hybridization

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