Depolarization and cAMP elevation rapidly recruit TrkB to the plasma membrane of CNS neurons

Anke Meyer-Franke, George A. Wilkinson, Alex Kruttgen, Minjie Hu, Elizabeth Munro, Martin G. Hanson, Louis F. Reichardt, Ben A. Barres

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Abstract

Here, we describe a novel mechanism for the rapid regulation of surface levels of the neurotrophin reCeptor TrkB. Unlike nodose ganglion neurons, both retinal ganglion cells (RGCs) and spinal motor neurons (SMNs) in culture display only low levels of surface TrkB, though high levels are present intracellularly. Within minutes of depolarization or cAMP elevation, surface TrkB levels increase by nearly 4-fold, and this increase is not blocked by cycloheximide. These findings suggest that activity and cAMP elevation rapidly recruit TrkB to the plasma membrane by translocation from intracellular stores. We propose that a fundamental difference between peripheral nervous system (PNS) and central nervous system (CNS) neurons is the activity dependence of CNS neurons for responsiveness to their peptide trophic factors and that differences in membrane compartmentalization of the receptors underlie this difference.

Original languageEnglish (US)
Pages (from-to)681-693
Number of pages13
JournalNeuron
Volume21
Issue number4
DOIs
StatePublished - Oct 1998

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ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Meyer-Franke, A., Wilkinson, G. A., Kruttgen, A., Hu, M., Munro, E., Hanson, M. G., Reichardt, L. F., & Barres, B. A. (1998). Depolarization and cAMP elevation rapidly recruit TrkB to the plasma membrane of CNS neurons. Neuron, 21(4), 681-693. https://doi.org/10.1016/S0896-6273(00)80586-3