Demonstration of tyrosinase in the adult bovine uveal tract and retinal pigment epithelium

T. P. Dryja, M. O'Neil-Dryja, J. M. Pawelek, D. M. Albert

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

Modern techniques offer an opportunity for a more complete evaluation of melanin production in the uvea and retinal pigment epithelium (RPE). By measuring the release of tritium from tritiated tyrosine in homogenized samples of adult bovine RPE as well as iris and choroid, tyrosinase activity could be demonstrated in both the uveal tract and the RPE. Phenylthiourea, a specific tyrosinase inhibitor, markedly decreased tyrosinase activity, whereas 3-iodo-tyrosine, a tyrosine hydroxylase inhibitor, had no effect. These techniques indicate tyrosinase activity in the uveal tract and the RPE of adult cattle. This is the first biochemical demonstration of tyrosinase in adult RPE.

Original languageEnglish (US)
Pages (from-to)511-514
Number of pages4
JournalInvestigative Ophthalmology and Visual Science
Volume17
Issue number6
StatePublished - Jan 1 1978

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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