Demonstration of insulin-like growth factor (IGF-I and -II) receptors and binding protein in human breast cancer cell lines

D. D. De Leon, B. Bakker, D. M. Wilson, R. L. Hintz, Ronald (Ron) Rosenfeld

Research output: Contribution to journalArticle

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Abstract

The insulin like growth factors (IGFs), potent mitogens for a variety of normal and transformed cells, have been reported to be secreted by several human breast cancer cell lines (BC). We have investigated the binding characteristics of IGF-I and -II in four human BC: MCF-7, T-47D, MDA 231 and Hs578T. Binding studies in microsomal membrane preparations detected high specific binding for both IGF in all four BC studied. Cross-linking with 125I-IGF-I, followed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) under reduced conditions, revealed the presence of an α subunit of apparent Mr = 130,000 in MCF-7, T-47D and MDA 213 cells. When 125I-IGF-II was cross-linked, a major band of apparent Mr = 260,000 was seen in all BC. This band was inhibited by IGF-II, but not by insulin. Cross-linking of 125I-IGF-I to conditioned media from BC demonstrated the presence of three binding proteins of apparent Mr = 45,000, 36,000 and 29,000 in all BC but T-47D, in which the 36,000 band was not seen. These data demonstrate that BC possess classical receptors for both IGF-I and -II and, furthermore, that BC produce specific binding proteins for these growth factors.

Original languageEnglish (US)
Pages (from-to)398-405
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume152
Issue number1
DOIs
StatePublished - Apr 15 1988
Externally publishedYes

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IGF Type 2 Receptor
IGF Type 1 Receptor
Insulin-Like Growth Factor II
Somatomedins
Insulin-Like Growth Factor I
Carrier Proteins
Demonstrations
Cells
Breast Neoplasms
Cell Line
Conditioned Culture Medium
Electrophoresis
Mitogens
Sodium Dodecyl Sulfate
Intercellular Signaling Peptides and Proteins
Insulin
Membranes
Polyacrylamide Gel Electrophoresis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Demonstration of insulin-like growth factor (IGF-I and -II) receptors and binding protein in human breast cancer cell lines. / De Leon, D. D.; Bakker, B.; Wilson, D. M.; Hintz, R. L.; Rosenfeld, Ronald (Ron).

In: Biochemical and Biophysical Research Communications, Vol. 152, No. 1, 15.04.1988, p. 398-405.

Research output: Contribution to journalArticle

De Leon, D. D. ; Bakker, B. ; Wilson, D. M. ; Hintz, R. L. ; Rosenfeld, Ronald (Ron). / Demonstration of insulin-like growth factor (IGF-I and -II) receptors and binding protein in human breast cancer cell lines. In: Biochemical and Biophysical Research Communications. 1988 ; Vol. 152, No. 1. pp. 398-405.
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