Delayed localization of synelfin (synuclein, NACP) to presynaptic terminals in cultured rat hippocampal neurons

Ginger S. Withers, Julia M. George, Gary A. Banker, David F. Clayton

Research output: Contribution to journalArticle

170 Scopus citations

Abstract

Synelfin is a presynaptic protein of unknown function that is differentially regulated in the avian song control circuit during the critical period for song learning; in humans, it gives rise to an amyloidogenic peptide found in senile plaques of Alzheimer's disease. To gain insight into the potential involvement of synelfin in synapse development, we investigated its expression in neurons cultured from the embryonic rat hippocampus. These neurons express a variety of defined synaptic proteins, and form numerous synaptic connections after several days in culture. Synapsin I, a synaptic vesicle-associated protein, was detected within one day after the neurons were put in culture, but significant immunoreactivity for synelfin was not detected until ~ 5 days in vitro (DIV). By 3 DIV, synapsin-positive puncta (previously shown to correspond to presynaptic specializations) were detected surrounding the soma and proximal dendritic processes, whereas comparable aggregations of synelfin did not appear until several days later. By 14 DIV the punctate concentrations of synelfin and synapsin overlapped completely. Thus synelfin is expressed in these cultured neurons and eventually becomes localized to presynaptic terminals, but it is absent from these specializations when they first form. We conclude that presynaptic terminals can change in molecular composition, and that synelfin is associated with later stages in synaptic development or modulation.

Original languageEnglish (US)
Pages (from-to)87-94
Number of pages8
JournalDevelopmental Brain Research
Volume99
Issue number1
DOIs
StatePublished - Mar 17 1997

Keywords

  • non-amyloid component precursor
  • presynaptic terminal
  • rat hippocampal culture
  • synaptic maturation
  • synaptogenesis
  • synelfin
  • synuclein

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Developmental Biology

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