Defective calmodulin-dependent rapid apical endocytosis in zebrafish sensory hair cell mutants

Christoph Seiler, Teresa Nicolson

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

Vertebrate mechanosensory hair cells contain a narrow 'pericuticular' zone which is densely populated with small vesicles between the cuticular plate and cellular junctions near the apical surface. The presence of many cytoplasmic vesicles suggests that the apical surface of hair cells has a high turnover rate. The significance of intense membrane trafficking at the apical surface is not known. Using a marker of endocytosis, the styryl dye FM1-43, this report shows that rapid apical endocytosis in zebrafish lateral line sensory hair cells is calcium and calmodulin dependent and is partially blocked by the presence of amiloride and dihydrostreptomycin, known inhibitors of mechanotransduction channels. As seen in lateral line hair cells, sensory hair cells within the larval otic capsule also exhibit rapid apical endocytosis. Defects in internalization of the dye in both lateral line and inner ear hair cells were found in five zebrafish auditory/vestibular mutants: sputnik, mariner, orbiter, mercury, and skylab. In addition, lateral line hair cells in these mutants were not sensitive to prolonged exposure to streptomycin, which is toxic to hair cells. The presence of endocytic defects in the majority of zebrafish mechanosensory mutants points to a important role of apical endocytosis in hair cell function.

Original languageEnglish (US)
Pages (from-to)424-434
Number of pages11
JournalJournal of Neurobiology
Volume41
Issue number3
DOIs
StatePublished - Nov 15 1999
Externally publishedYes

Fingerprint

Zebrafish
Calmodulin
Endocytosis
Inner Auditory Hair Cells
Coloring Agents
Dihydrostreptomycin Sulfate
Cytoplasmic Vesicles
Amiloride
Poisons
Streptomycin
Inner Ear
Mercury
Capsules
Ear
Vertebrates
Calcium
Membranes

Keywords

  • Endocytosis
  • FMI-43
  • Ototoxicity
  • Sensory hair cells
  • Zebrafish

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Defective calmodulin-dependent rapid apical endocytosis in zebrafish sensory hair cell mutants. / Seiler, Christoph; Nicolson, Teresa.

In: Journal of Neurobiology, Vol. 41, No. 3, 15.11.1999, p. 424-434.

Research output: Contribution to journalArticle

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