Decreased inhibin B secretion is associated with the monotropic FSH rise in older, ovulatory women: A study of serum and follicular fluid levels of dimeric inhibin A and B in spontaneous menstrual cycles

N. A. Klein, P. J. Illingworth, N. P. Groome, A. S. McNeilly, David Battaglia, M. R. Soules

Research output: Contribution to journalArticle

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Abstract

This study sought to compare circulating and follicular fluid (FF) concentrations of dimeric inhibin A and B utilizing specific two-site ELISAs for these hormones in normal older and younger ovulatory women. Methods: Normally ovulating women age 40-45 (n=10) and 20-25 (n=13) were studied throughout the follicular phase with daily blood sampling, transvaginal ultrasound examinations, and dominant follicle aspiration. When the dominant follicle reached a mean diameter of 16 mm or serum estradiol (E2) was ≤550 pmol/L, 10,000 IU of hCG was administered intramuscularly followed 32 hours later by transvaginal follicle aspiration. Serum and FF samples were analyzed for E2, FSH, and inhibin A and B. Daily hormone levels were compared by ANOVA, and mean results were compared using t-tests. Results: Older women developed a dominant follicle sooner, meeting criteria for hCG cycle day 10.6±0.4 vs.14.5±1.0 p2 concentration was higher in the older women (p=0.002), and there was no significant difference in inhibin A secretion (p=0.61). In contrast, mean inhibin B concentration was significantly lower in the older women (p=0.04). On the day of aspiration of the dominant follicle, serum inhibin B was decreased in the older subjects (42.6±6.5 vs. 153.1±53 pg/ml, p=0.02), whereas older subjects had higher levels of inhibin A (106±16 vs. 60.4±9.4 pg/ml, p=0.04) and similar E2 levels (665±35.2 vs. 687±92 pmol/L, p=0.83). There were no differences in FF concentrations of inhibin B (164±31 vs. 174±37 ng/ml, p=0.85), inhibin A (317.7±38 vs. 248±57 ng/ml, p=0.16), or E2 (2074±294 vs. 2474±338 nmol/L, p=0.82) in the older and younger women. Conclusion: Follicular phase inhibin B secretion is decreased in older ovulatory women who demonstrate a monotropic FSH rise, whereas inhibin A secretion is similar to that in younger women. The dominant follicle in these older women appears to be normal in terms of FF E2 and inhibin content. We speculate that decreased inhibin B secretion most likely reflects a diminished follicular pool in older women and may be an important regulator of the monotropic FSH rise.

Original languageEnglish (US)
Pages (from-to)2742-2745
Number of pages4
JournalJournal of Clinical Endocrinology and Metabolism
Volume81
Issue number7
DOIs
StatePublished - 1996
Externally publishedYes

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Follicular Fluid
Menstrual Cycle
Fluids
Serum
Follicular Phase
Hormones
Inhibins
Analysis of variance (ANOVA)
inhibin B
inhibin A
Estradiol
Blood
Ultrasonics
Sampling
Analysis of Variance
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Decreased inhibin B secretion is associated with the monotropic FSH rise in older, ovulatory women : A study of serum and follicular fluid levels of dimeric inhibin A and B in spontaneous menstrual cycles. / Klein, N. A.; Illingworth, P. J.; Groome, N. P.; McNeilly, A. S.; Battaglia, David; Soules, M. R.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 81, No. 7, 1996, p. 2742-2745.

Research output: Contribution to journalArticle

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title = "Decreased inhibin B secretion is associated with the monotropic FSH rise in older, ovulatory women: A study of serum and follicular fluid levels of dimeric inhibin A and B in spontaneous menstrual cycles",
abstract = "This study sought to compare circulating and follicular fluid (FF) concentrations of dimeric inhibin A and B utilizing specific two-site ELISAs for these hormones in normal older and younger ovulatory women. Methods: Normally ovulating women age 40-45 (n=10) and 20-25 (n=13) were studied throughout the follicular phase with daily blood sampling, transvaginal ultrasound examinations, and dominant follicle aspiration. When the dominant follicle reached a mean diameter of 16 mm or serum estradiol (E2) was ≤550 pmol/L, 10,000 IU of hCG was administered intramuscularly followed 32 hours later by transvaginal follicle aspiration. Serum and FF samples were analyzed for E2, FSH, and inhibin A and B. Daily hormone levels were compared by ANOVA, and mean results were compared using t-tests. Results: Older women developed a dominant follicle sooner, meeting criteria for hCG cycle day 10.6±0.4 vs.14.5±1.0 p2 concentration was higher in the older women (p=0.002), and there was no significant difference in inhibin A secretion (p=0.61). In contrast, mean inhibin B concentration was significantly lower in the older women (p=0.04). On the day of aspiration of the dominant follicle, serum inhibin B was decreased in the older subjects (42.6±6.5 vs. 153.1±53 pg/ml, p=0.02), whereas older subjects had higher levels of inhibin A (106±16 vs. 60.4±9.4 pg/ml, p=0.04) and similar E2 levels (665±35.2 vs. 687±92 pmol/L, p=0.83). There were no differences in FF concentrations of inhibin B (164±31 vs. 174±37 ng/ml, p=0.85), inhibin A (317.7±38 vs. 248±57 ng/ml, p=0.16), or E2 (2074±294 vs. 2474±338 nmol/L, p=0.82) in the older and younger women. Conclusion: Follicular phase inhibin B secretion is decreased in older ovulatory women who demonstrate a monotropic FSH rise, whereas inhibin A secretion is similar to that in younger women. The dominant follicle in these older women appears to be normal in terms of FF E2 and inhibin content. We speculate that decreased inhibin B secretion most likely reflects a diminished follicular pool in older women and may be an important regulator of the monotropic FSH rise.",
author = "Klein, {N. A.} and Illingworth, {P. J.} and Groome, {N. P.} and McNeilly, {A. S.} and David Battaglia and Soules, {M. R.}",
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T1 - Decreased inhibin B secretion is associated with the monotropic FSH rise in older, ovulatory women

T2 - A study of serum and follicular fluid levels of dimeric inhibin A and B in spontaneous menstrual cycles

AU - Klein, N. A.

AU - Illingworth, P. J.

AU - Groome, N. P.

AU - McNeilly, A. S.

AU - Battaglia, David

AU - Soules, M. R.

PY - 1996

Y1 - 1996

N2 - This study sought to compare circulating and follicular fluid (FF) concentrations of dimeric inhibin A and B utilizing specific two-site ELISAs for these hormones in normal older and younger ovulatory women. Methods: Normally ovulating women age 40-45 (n=10) and 20-25 (n=13) were studied throughout the follicular phase with daily blood sampling, transvaginal ultrasound examinations, and dominant follicle aspiration. When the dominant follicle reached a mean diameter of 16 mm or serum estradiol (E2) was ≤550 pmol/L, 10,000 IU of hCG was administered intramuscularly followed 32 hours later by transvaginal follicle aspiration. Serum and FF samples were analyzed for E2, FSH, and inhibin A and B. Daily hormone levels were compared by ANOVA, and mean results were compared using t-tests. Results: Older women developed a dominant follicle sooner, meeting criteria for hCG cycle day 10.6±0.4 vs.14.5±1.0 p2 concentration was higher in the older women (p=0.002), and there was no significant difference in inhibin A secretion (p=0.61). In contrast, mean inhibin B concentration was significantly lower in the older women (p=0.04). On the day of aspiration of the dominant follicle, serum inhibin B was decreased in the older subjects (42.6±6.5 vs. 153.1±53 pg/ml, p=0.02), whereas older subjects had higher levels of inhibin A (106±16 vs. 60.4±9.4 pg/ml, p=0.04) and similar E2 levels (665±35.2 vs. 687±92 pmol/L, p=0.83). There were no differences in FF concentrations of inhibin B (164±31 vs. 174±37 ng/ml, p=0.85), inhibin A (317.7±38 vs. 248±57 ng/ml, p=0.16), or E2 (2074±294 vs. 2474±338 nmol/L, p=0.82) in the older and younger women. Conclusion: Follicular phase inhibin B secretion is decreased in older ovulatory women who demonstrate a monotropic FSH rise, whereas inhibin A secretion is similar to that in younger women. The dominant follicle in these older women appears to be normal in terms of FF E2 and inhibin content. We speculate that decreased inhibin B secretion most likely reflects a diminished follicular pool in older women and may be an important regulator of the monotropic FSH rise.

AB - This study sought to compare circulating and follicular fluid (FF) concentrations of dimeric inhibin A and B utilizing specific two-site ELISAs for these hormones in normal older and younger ovulatory women. Methods: Normally ovulating women age 40-45 (n=10) and 20-25 (n=13) were studied throughout the follicular phase with daily blood sampling, transvaginal ultrasound examinations, and dominant follicle aspiration. When the dominant follicle reached a mean diameter of 16 mm or serum estradiol (E2) was ≤550 pmol/L, 10,000 IU of hCG was administered intramuscularly followed 32 hours later by transvaginal follicle aspiration. Serum and FF samples were analyzed for E2, FSH, and inhibin A and B. Daily hormone levels were compared by ANOVA, and mean results were compared using t-tests. Results: Older women developed a dominant follicle sooner, meeting criteria for hCG cycle day 10.6±0.4 vs.14.5±1.0 p2 concentration was higher in the older women (p=0.002), and there was no significant difference in inhibin A secretion (p=0.61). In contrast, mean inhibin B concentration was significantly lower in the older women (p=0.04). On the day of aspiration of the dominant follicle, serum inhibin B was decreased in the older subjects (42.6±6.5 vs. 153.1±53 pg/ml, p=0.02), whereas older subjects had higher levels of inhibin A (106±16 vs. 60.4±9.4 pg/ml, p=0.04) and similar E2 levels (665±35.2 vs. 687±92 pmol/L, p=0.83). There were no differences in FF concentrations of inhibin B (164±31 vs. 174±37 ng/ml, p=0.85), inhibin A (317.7±38 vs. 248±57 ng/ml, p=0.16), or E2 (2074±294 vs. 2474±338 nmol/L, p=0.82) in the older and younger women. Conclusion: Follicular phase inhibin B secretion is decreased in older ovulatory women who demonstrate a monotropic FSH rise, whereas inhibin A secretion is similar to that in younger women. The dominant follicle in these older women appears to be normal in terms of FF E2 and inhibin content. We speculate that decreased inhibin B secretion most likely reflects a diminished follicular pool in older women and may be an important regulator of the monotropic FSH rise.

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