Decrease in salivary secretion by radiation mediated by nitric oxide and prostaglandins

Carolina De La Cal, Alejandro Lomniczi, Claudia E. Mohn, Andrea De Laurentiis, Mariana Casal, Ana Chiarenza, Dante Paz, Samuel M. McCann, Valeria Rettori, Juan Carlos Elverdín

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Objective: In the present work, we evaluated the effect of exposing the submandibular glands (SMG) to radiation, studying different functional parameters such as salivary secretion, nitric oxide (NO) production, reactive oxygen species formation, prostaglandin (PGE) content and apoptosis. Methods: We irradiated rats in the head and neck region with a single dose of γ-ray radiation of 15 Gy. Two hours after radiation, we measured norepinephrine- induced salivary secretion. After that, the SMG were dissected, and in this tissue, we measured the activity of NO synthase (NOS), the PGE content, the amount of reactive oxygen species, apoptotic cells and mitochondrial inducible NOS (iNOS) expression. Results: We found that radiation decreased salivary secretion when 10 and 30 μg/kg of norepinephrine was administered via the right femoral vein. We observed that iNOS activity was reduced and PGE content increased after radiation in SMG, indicating that NO and PGEs may participate in salivary secretion. The expression of mitochondrial NOS was increased after radiation leading to the formation of large amounts of NO that acts as a proapoptotic signal. In fact, we observed an augmentation in apoptotic cells. In this study, we also observed an increase in lipid peroxidation induced by radiation that may contribute to tissue damage. Conclusions: Our results indicate that radiation induced a decrease in salivary secretion and SMG iNOS activity, meanwhile the PGE content, the lipid peroxidation and apoptosis increased in the tissue. These modifications decrease salivary secretion.

Original languageEnglish (US)
Pages (from-to)19-27
Number of pages9
JournalNeuroImmunoModulation
Volume13
Issue number1
DOIs
Publication statusPublished - Aug 2006
Externally publishedYes

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Keywords

  • Apoptosis
  • Nitric oxide synthase
  • Submandibular glands
  • Xerostomia

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Immunology

Cite this

De La Cal, C., Lomniczi, A., Mohn, C. E., De Laurentiis, A., Casal, M., Chiarenza, A., ... Elverdín, J. C. (2006). Decrease in salivary secretion by radiation mediated by nitric oxide and prostaglandins. NeuroImmunoModulation, 13(1), 19-27. https://doi.org/10.1159/000093194