Decrease in annexin I messenger ribonucleic acid expression in human amnion with labor

David I. Lynch-Salamon, William V. Everson, Leslie Myatt

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

OBJECTIVE: Annexins are a superfamily of proteins that are thought to inhibit phospholipase A2 activity and hence inhibit prostaglandin production. The purpose of this study was to test the hypothesis that annexin I concentration in human amnion is reduced with labor and that this reduction is mediated by a decrease in annexin I messenger ribonucleic acid expression. STUDY DESIGN: Amnion and choriodecidua were collected from term singleton pregnancies, eight after spontaneous vaginal delivery and eight from elective cesarean section without labor. Annexin I protein was quantitated by Western blotting. Ribonucleic acid was isolated from amnion, and then annexin I messenger ribonucleic acid was identified by Northern hybridization and quantitated by slot blotting. RESULTS: Annexin I (35 kd) was identified in amnion tissue. The concentration in the group undergoing labor (320 ± 45 integrated optical density units, mean ± SE) was significantly reduced (p <0.05) compared with that in the group not undergoing labor (635 ± 65 units). The size of the annexin I messenger ribonucleic acid was approximately 1.8 kb. The mean integrated optical density for the labor group (840 ± 139 units, mean ± SE) was significantly reduced (p <0.05) compared with that of the nonlabor group (1912 ± 464 units). CONCLUSION: There is a significant decrease in annexin I messenger ribonucleic acid expression in human amnion with labor, corresponding to a significant decrease in annexin I protein concentration. This may contribute to the increased phospholipase A+2 activity, arachidonic acid mobilization, and prostaglandin production at labor in humans.

Original languageEnglish (US)
Pages (from-to)1657-1663
Number of pages7
JournalAmerican Journal of Obstetrics and Gynecology
Volume167
Issue number6
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Annexin A1
Amnion
RNA
Prostaglandins
Annexins
Proteins
Phospholipases A
Phospholipases A2
Arachidonic Acid
Cesarean Section
Western Blotting
Pregnancy

Keywords

  • amnion
  • Annexin
  • labor
  • phospholipase A
  • prostaglandin

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Decrease in annexin I messenger ribonucleic acid expression in human amnion with labor. / Lynch-Salamon, David I.; Everson, William V.; Myatt, Leslie.

In: American Journal of Obstetrics and Gynecology, Vol. 167, No. 6, 1992, p. 1657-1663.

Research output: Contribution to journalArticle

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N2 - OBJECTIVE: Annexins are a superfamily of proteins that are thought to inhibit phospholipase A2 activity and hence inhibit prostaglandin production. The purpose of this study was to test the hypothesis that annexin I concentration in human amnion is reduced with labor and that this reduction is mediated by a decrease in annexin I messenger ribonucleic acid expression. STUDY DESIGN: Amnion and choriodecidua were collected from term singleton pregnancies, eight after spontaneous vaginal delivery and eight from elective cesarean section without labor. Annexin I protein was quantitated by Western blotting. Ribonucleic acid was isolated from amnion, and then annexin I messenger ribonucleic acid was identified by Northern hybridization and quantitated by slot blotting. RESULTS: Annexin I (35 kd) was identified in amnion tissue. The concentration in the group undergoing labor (320 ± 45 integrated optical density units, mean ± SE) was significantly reduced (p <0.05) compared with that in the group not undergoing labor (635 ± 65 units). The size of the annexin I messenger ribonucleic acid was approximately 1.8 kb. The mean integrated optical density for the labor group (840 ± 139 units, mean ± SE) was significantly reduced (p <0.05) compared with that of the nonlabor group (1912 ± 464 units). CONCLUSION: There is a significant decrease in annexin I messenger ribonucleic acid expression in human amnion with labor, corresponding to a significant decrease in annexin I protein concentration. This may contribute to the increased phospholipase A+2 activity, arachidonic acid mobilization, and prostaglandin production at labor in humans.

AB - OBJECTIVE: Annexins are a superfamily of proteins that are thought to inhibit phospholipase A2 activity and hence inhibit prostaglandin production. The purpose of this study was to test the hypothesis that annexin I concentration in human amnion is reduced with labor and that this reduction is mediated by a decrease in annexin I messenger ribonucleic acid expression. STUDY DESIGN: Amnion and choriodecidua were collected from term singleton pregnancies, eight after spontaneous vaginal delivery and eight from elective cesarean section without labor. Annexin I protein was quantitated by Western blotting. Ribonucleic acid was isolated from amnion, and then annexin I messenger ribonucleic acid was identified by Northern hybridization and quantitated by slot blotting. RESULTS: Annexin I (35 kd) was identified in amnion tissue. The concentration in the group undergoing labor (320 ± 45 integrated optical density units, mean ± SE) was significantly reduced (p <0.05) compared with that in the group not undergoing labor (635 ± 65 units). The size of the annexin I messenger ribonucleic acid was approximately 1.8 kb. The mean integrated optical density for the labor group (840 ± 139 units, mean ± SE) was significantly reduced (p <0.05) compared with that of the nonlabor group (1912 ± 464 units). CONCLUSION: There is a significant decrease in annexin I messenger ribonucleic acid expression in human amnion with labor, corresponding to a significant decrease in annexin I protein concentration. This may contribute to the increased phospholipase A+2 activity, arachidonic acid mobilization, and prostaglandin production at labor in humans.

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