Cyclic AMP stabilizes the degradation of original junctional acetylcholine receptors in denervated muscle

Show-Ling Shyng, R. Xu, M. M. Salpeter

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

We used mouse diaphragm muscle in organ culture to study the stabilization of acetylcholine receptor (AChR) degradation at denervated neuromuscular junctions. After denervation, the degradation rate of the AChRs present prior to denervation (slowly degrading, or Rs, AChRs) accelerates from the predenervation degradation half-life (t 1 2) of ∼8-10 days to a t 1 2 of ∼2-3 days. We report that addition to the organ culture medium of pharmacological agents that elevate cytoplasmic cAMP levels (forskolin, dibutyryl cAMP, and 8-bromo-cAMP) reversed the change in 42 caused by denervation, whereas addition of 1,9-dideoxyforskolin, a forskolin analog that does not elevate cytoplasmic cAMP levels, did not reverse the effect of denervation. The degradation rate of AChRs in primary myotube cultures and that of the newly synthesized AChRs in denervated muscle were little affected by forskolin or dibutyryl cAMP. The possibility is raised that the modulation of Its AChR degradation by innervation may be mediated by CAMP.

Original languageEnglish (US)
Pages (from-to)469-475
Number of pages7
JournalNeuron
Volume6
Issue number3
DOIs
StatePublished - 1991
Externally publishedYes

Fingerprint

Cholinergic Receptors
Denervation
Cyclic AMP
Colforsin
Muscles
Organ Culture Techniques
8-Bromo Cyclic Adenosine Monophosphate
Neuromuscular Junction
Skeletal Muscle Fibers
Diaphragm
Half-Life
Culture Media
Pharmacology

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cyclic AMP stabilizes the degradation of original junctional acetylcholine receptors in denervated muscle. / Shyng, Show-Ling; Xu, R.; Salpeter, M. M.

In: Neuron, Vol. 6, No. 3, 1991, p. 469-475.

Research output: Contribution to journalArticle

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