Cultured human brain capillary endothelial cells are permissive for infection by human cytomegalovirus

Janet L. Lathey; Clayton A. Wiley; M. Anthony Verity; Jay A. Nelson

doi:10.1016/0042-6822(90)90252-M

Cultured human brain capillary endothelial cells are permissive for infection by human cytomegalovirus

Janet L. Lathey, Clayton A. Wiley, M. Anthony Verity, Jay A. Nelson

Research output: Contribution to journal › Article › peer-review

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Abstract

Human cytomegalovirus (HCMV) infection is associated with a variety of systemic and neurologic diseases.In vitro HCMV growth is usually studied in fibroblasts, while in vivo HCMV growth is frequently observed in a wide variety of cell types including glia, neurons, and human brain capillary endothelial (HBCE) cells. To examine the biology of HCMV in HBCE cells, we have established a procedure for isolating these cells from human brain temporal lobectomies. Greater than 99.0% of these cultured cells were identified as HBCE cells on the basis of positive staining for factor VIII-related antigen-Von Willebrand's factor (F VIII) and Ulex Europaeus agglutinin I (UEA I[t HCMV antigens were detected by immunocytochemistry in HBCE cells after infection with strain AD 169. Intracellular virions were observed in infected cells by electron microscopy and infectious virus was released from HBCE cells. In addition, infected cells were confirmed as endothelial cells by double staining with antibodies to F VIII and HCMV.

Original language	English (US)
Pages (from-to)	266-273
Number of pages	8
Journal	Virology
Volume	176
Issue number	1
DOIs	https://doi.org/10.1016/0042-6822(90)90252-M
State	Published - May 1990
Externally published	Yes

ASJC Scopus subject areas

Virology

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10.1016/0042-6822(90)90252-M

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title = "Cultured human brain capillary endothelial cells are permissive for infection by human cytomegalovirus",

abstract = "Human cytomegalovirus (HCMV) infection is associated with a variety of systemic and neurologic diseases.In vitro HCMV growth is usually studied in fibroblasts, while in vivo HCMV growth is frequently observed in a wide variety of cell types including glia, neurons, and human brain capillary endothelial (HBCE) cells. To examine the biology of HCMV in HBCE cells, we have established a procedure for isolating these cells from human brain temporal lobectomies. Greater than 99.0% of these cultured cells were identified as HBCE cells on the basis of positive staining for factor VIII-related antigen-Von Willebrand's factor (F VIII) and Ulex Europaeus agglutinin I (UEA I[t HCMV antigens were detected by immunocytochemistry in HBCE cells after infection with strain AD 169. Intracellular virions were observed in infected cells by electron microscopy and infectious virus was released from HBCE cells. In addition, infected cells were confirmed as endothelial cells by double staining with antibodies to F VIII and HCMV.",

author = "Lathey, {Janet L.} and Wiley, {Clayton A.} and Verity, {M. Anthony} and Nelson, {Jay A.}",

note = "Funding Information: This is Publication 5902.IMM from the Department of Immunology, Scripps Clinic and Research Foundatron (La Jolla. CA). Thiswork was supported in part by USPHS Grants Al-24178 AI-21 640, and AG-04342 (J.A.N.), NIH NINCDS NS-00928, NS-251781, NS-27417, and AIDS Task Force (C.A.W.), and NIH Postdoctoral Training Award NS-07078 to J.L.L. J.A.N. IS the recipient of a faculty award from the American Cancer Society. The authors thank Rick Landes and G. Patrick Burrola for their technical assistance and Margaret Stone for manuscript preparation.",

year = "1990",

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doi = "10.1016/0042-6822(90)90252-M",

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AU - Wiley, Clayton A.

AU - Verity, M. Anthony

AU - Nelson, Jay A.

N1 - Funding Information: This is Publication 5902.IMM from the Department of Immunology, Scripps Clinic and Research Foundatron (La Jolla. CA). Thiswork was supported in part by USPHS Grants Al-24178 AI-21 640, and AG-04342 (J.A.N.), NIH NINCDS NS-00928, NS-251781, NS-27417, and AIDS Task Force (C.A.W.), and NIH Postdoctoral Training Award NS-07078 to J.L.L. J.A.N. IS the recipient of a faculty award from the American Cancer Society. The authors thank Rick Landes and G. Patrick Burrola for their technical assistance and Margaret Stone for manuscript preparation.

PY - 1990/5

Y1 - 1990/5

N2 - Human cytomegalovirus (HCMV) infection is associated with a variety of systemic and neurologic diseases.In vitro HCMV growth is usually studied in fibroblasts, while in vivo HCMV growth is frequently observed in a wide variety of cell types including glia, neurons, and human brain capillary endothelial (HBCE) cells. To examine the biology of HCMV in HBCE cells, we have established a procedure for isolating these cells from human brain temporal lobectomies. Greater than 99.0% of these cultured cells were identified as HBCE cells on the basis of positive staining for factor VIII-related antigen-Von Willebrand's factor (F VIII) and Ulex Europaeus agglutinin I (UEA I[t HCMV antigens were detected by immunocytochemistry in HBCE cells after infection with strain AD 169. Intracellular virions were observed in infected cells by electron microscopy and infectious virus was released from HBCE cells. In addition, infected cells were confirmed as endothelial cells by double staining with antibodies to F VIII and HCMV.

AB - Human cytomegalovirus (HCMV) infection is associated with a variety of systemic and neurologic diseases.In vitro HCMV growth is usually studied in fibroblasts, while in vivo HCMV growth is frequently observed in a wide variety of cell types including glia, neurons, and human brain capillary endothelial (HBCE) cells. To examine the biology of HCMV in HBCE cells, we have established a procedure for isolating these cells from human brain temporal lobectomies. Greater than 99.0% of these cultured cells were identified as HBCE cells on the basis of positive staining for factor VIII-related antigen-Von Willebrand's factor (F VIII) and Ulex Europaeus agglutinin I (UEA I[t HCMV antigens were detected by immunocytochemistry in HBCE cells after infection with strain AD 169. Intracellular virions were observed in infected cells by electron microscopy and infectious virus was released from HBCE cells. In addition, infected cells were confirmed as endothelial cells by double staining with antibodies to F VIII and HCMV.

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Cultured human brain capillary endothelial cells are permissive for infection by human cytomegalovirus

Abstract

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