Cultivation of mesophilic soil crenarchaeotes in enrichment cultures from plant roots

Holly Simon, Courtney E. Jahn, Luke T. Bergerud, Marek K. Sliwinski, Paul J. Weimer, David K. Willis, Robert M. Goodman

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Because archaea are generally associated with extreme environments, detection of nonthermophilic members belonging to the archaeal division Crenarchaeota over the last decade was unexpected; they are surprisingly ubiquitous and abundant in nonextreme marine and terrestrial habitats. Metabolic characterization of these nonthermophilic crenarchaeotes has been impeded by their intractability toward isolation and growth in culture. From studies employing a combination of cultivation and molecular phylogenetic techniques (PCR-single-strand conformation polymorphism, sequence analysis of 16S rRNA genes, fluorescence in situ hybridization, and real-time PCR), we present evidence here that one of the two dominant phylotypes of Crenarchaeota that colonizes the roots of tomato plants grown in soil from a Wisconsin field is selectively enriched in mixed cultures amended with root extract. Clones recovered from enrichment cultures were found to group phylogenetically with sequences from clade C1b.A1. This work corroborates and extends our recent findings, indicating that the diversity of the crenarchaeal soil assemblage is influenced by the rhizosphere and that mesophilic soil crenarchaeotes are found associated with plant roots, and provides the first evidence for growth of nonthermophilic crenarchaeotes in culture.

Original languageEnglish (US)
Pages (from-to)4751-4760
Number of pages10
JournalApplied and Environmental Microbiology
Volume71
Issue number8
DOIs
StatePublished - Aug 2005

Fingerprint

Crenarchaeota
enrichment culture
Plant Roots
tillage
Soil
Rhizosphere
soil
Archaea
Lycopersicon esculentum
Growth
Fluorescence In Situ Hybridization
rRNA Genes
Ecosystem
Sequence Analysis
Real-Time Polymerase Chain Reaction
Clone Cells
single-stranded conformational polymorphism
mixed culture
rhizosphere
clone

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Simon, H., Jahn, C. E., Bergerud, L. T., Sliwinski, M. K., Weimer, P. J., Willis, D. K., & Goodman, R. M. (2005). Cultivation of mesophilic soil crenarchaeotes in enrichment cultures from plant roots. Applied and Environmental Microbiology, 71(8), 4751-4760. https://doi.org/10.1128/AEM.71.8.4751-4760.2005

Cultivation of mesophilic soil crenarchaeotes in enrichment cultures from plant roots. / Simon, Holly; Jahn, Courtney E.; Bergerud, Luke T.; Sliwinski, Marek K.; Weimer, Paul J.; Willis, David K.; Goodman, Robert M.

In: Applied and Environmental Microbiology, Vol. 71, No. 8, 08.2005, p. 4751-4760.

Research output: Contribution to journalArticle

Simon, H, Jahn, CE, Bergerud, LT, Sliwinski, MK, Weimer, PJ, Willis, DK & Goodman, RM 2005, 'Cultivation of mesophilic soil crenarchaeotes in enrichment cultures from plant roots', Applied and Environmental Microbiology, vol. 71, no. 8, pp. 4751-4760. https://doi.org/10.1128/AEM.71.8.4751-4760.2005
Simon, Holly ; Jahn, Courtney E. ; Bergerud, Luke T. ; Sliwinski, Marek K. ; Weimer, Paul J. ; Willis, David K. ; Goodman, Robert M. / Cultivation of mesophilic soil crenarchaeotes in enrichment cultures from plant roots. In: Applied and Environmental Microbiology. 2005 ; Vol. 71, No. 8. pp. 4751-4760.
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