Crystallization of the α-hemolysin heptamer solubilized in decyldimethyl- and decyldiethylphosphine oxide

Langzhou Song, Eric Gouaux

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Crystals of the α-hemolysin heptamer, a transmembrane poreforming toxin from Staphylococcus aureus, have been grown using the nonionic detergents n-decyldimethyl- and n-decyldiethylphosphine oxide, phosphorus homologs of the ionic amine oxide detergents. Five crystal forms were obtained, one of which diffracted X-rays to 3 Å resolution. This crystal form displayed elements of pseudo mm symmetry in screened precession photographs yet it was triclinic with unit-cell dimensions a = 173, b = 173, c = 102 Å, α = 92.6, β = 94.8, γ = 90.2°.

Original languageEnglish (US)
Pages (from-to)276-278
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume54
Issue number2
DOIs
StatePublished - Mar 1 1998

ASJC Scopus subject areas

  • Structural Biology

Fingerprint Dive into the research topics of 'Crystallization of the α-hemolysin heptamer solubilized in decyldimethyl- and decyldiethylphosphine oxide'. Together they form a unique fingerprint.

  • Cite this