Crystallization of the α-hemolysin heptamer solubilized in decyldimethyl- and decyldiethylphosphine oxide

Langzhou Song, Eric Gouaux

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Crystals of the α-hemolysin heptamer, a transmembrane poreforming toxin from Staphylococcus aureus, have been grown using the nonionic detergents n-decyldimethyl- and n-decyldiethylphosphine oxide, phosphorus homologs of the ionic amine oxide detergents. Five crystal forms were obtained, one of which diffracted X-rays to 3 Å resolution. This crystal form displayed elements of pseudo mm symmetry in screened precession photographs yet it was triclinic with unit-cell dimensions a = 173, b = 173, c = 102 Å, α = 92.6, β = 94.8, γ = 90.2°.

Original languageEnglish (US)
Pages (from-to)276-278
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume54
Issue number2
DOIs
StatePublished - Mar 1 1998
Externally publishedYes

Fingerprint

Hemolysin Proteins
Crystallization
Detergents
detergents
crystallization
Crystals
oxides
phosphorus oxides
Phosphorus
Oxides
crystals
Amines
Staphylococcus aureus
staphylococcus
X-Rays
Crystal symmetry
photographs
precession
amines
X rays

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics
  • Structural Biology

Cite this

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abstract = "Crystals of the α-hemolysin heptamer, a transmembrane poreforming toxin from Staphylococcus aureus, have been grown using the nonionic detergents n-decyldimethyl- and n-decyldiethylphosphine oxide, phosphorus homologs of the ionic amine oxide detergents. Five crystal forms were obtained, one of which diffracted X-rays to 3 {\AA} resolution. This crystal form displayed elements of pseudo mm symmetry in screened precession photographs yet it was triclinic with unit-cell dimensions a = 173, b = 173, c = 102 {\AA}, α = 92.6, β = 94.8, γ = 90.2°.",
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AB - Crystals of the α-hemolysin heptamer, a transmembrane poreforming toxin from Staphylococcus aureus, have been grown using the nonionic detergents n-decyldimethyl- and n-decyldiethylphosphine oxide, phosphorus homologs of the ionic amine oxide detergents. Five crystal forms were obtained, one of which diffracted X-rays to 3 Å resolution. This crystal form displayed elements of pseudo mm symmetry in screened precession photographs yet it was triclinic with unit-cell dimensions a = 173, b = 173, c = 102 Å, α = 92.6, β = 94.8, γ = 90.2°.

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