Crystal structures of Toxoplasma gondii adenosine kinase reveal a novel catalytic mechanism and prodrug binding

Maria A. Schumacher, Daniel M. Scott, Iripam I. Mathews, Steven E. Ealick, David S. Roos, Buddy Ullman, Richard G. Brennan

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

Adenosine kinase (AK) is a key purine metabolic enzyme from the opportunistic parasitic protozoan Toxoplasma gondii and belongs to the family of carbohydrate kinases that includes ribokinase. To understand the catalytic mechanism of AK, we determined the structures of the T. gondii apo AK, AK:adenosine complex and the AK:adenosine:AMP-PCP complex to 2.55 Å, 2.50 Å and 1.71 Å resolution, respectively. These structures reveal a novel catalytic mechanism that involves an adenosine-induced domain rotation of 30°and a newly described anion hole (DTXGAGD), requiring a helix-to-coil conformational change that is induced by ATP binding. Nucleotide binding also evokes a coil-to-helix transition that completes the formation of the ATP binding pocket. A conserved dipeptide, Gly68-Gly69, which is located at the bottom of the adenosine-binding site, functions as the switch for domain rotation. The synergistic structural changes that occur upon substrate binding sequester the adenosine and the ATP gi phosphate from solvent and optimally position the substrates for catalysis. Finally, the 1.84 Å resolution structure of an AK:7-iodotubercidin:AMP-PCP complex reveals the basis for the higher affinity binding of this prodrug over adenosine and thus provides a scaffold for the design of new inhibitors and subversive substrates that target the T. gondii AK. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)549-567
Number of pages19
JournalJournal of molecular biology
Volume296
Issue number2
DOIs
StatePublished - Feb 18 2000

Keywords

  • Adenosine kinase
  • Drug design
  • Macromolecular crystallography
  • Purine
  • Toxoplasma gondii

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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    Schumacher, M. A., Scott, D. M., Mathews, I. I., Ealick, S. E., Roos, D. S., Ullman, B., & Brennan, R. G. (2000). Crystal structures of Toxoplasma gondii adenosine kinase reveal a novel catalytic mechanism and prodrug binding. Journal of molecular biology, 296(2), 549-567. https://doi.org/10.1006/jmbi.1999.3474