Cryptic human growth hormone gene sequences direct gonadotroph-specific expression in transgenic mice

M. J. Low, Richard Goodman, K. M. Ebert

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Our laboratory reported previously that chimeric genes encoding either rat somatostatin (SS) or human GH (hGH), but containing the identical mouse metallothionein-I (MT) promoter/enhancer sequences and hGH 3'-flanking sequences, were selectively expressed in the gonadotrophs of transgenic mice. The experiments reported here were designed to identify the DNA sequences responsible for this unexpected cell-specific expression within the anterior pituitary. We produced new transgenic mice expressing fusion genes that tested separately the requirement of the MT or 3'-hGH sequences for gonadotroph expression. A fusion gene that retained the original MT and SS sequences, with a simian virus 40 polyadenylation signal exchanged for the 3'-hGH sequences, no longer directed strong pituitary expression, but was active in the liver. In contrast, a cytomegalovirus promoter/enhancer-SS hGH fusion gene was expressed at the same high level in the anterior pituitaries of transgenic mice as the originally studied MT-SS-hGH gene. Immunohistochemical analysis indicated that pituitary expression of the cytomegalovirus promoter/enhancer-SS-hGH fusion gene was also restricted to gonadotroph cells in adult mice. These studies indicate that sequences within the 3'-flanking region of the hGH gene can direct expression of chimeric genes to pituitary cells that do not normally produce growth hormone.

Original languageEnglish (US)
Pages (from-to)2028-2033
Number of pages6
JournalMolecular Endocrinology
Volume3
Issue number12
StatePublished - 1989
Externally publishedYes

Fingerprint

Gonadotrophs
Human Growth Hormone
Transgenic Mice
Somatostatin
Metallothionein
Gene Fusion
Genes
3' Flanking Region
Cytomegalovirus
RNA 3' Polyadenylation Signals
Simian virus 40
Growth Hormone
Gene Expression
Liver

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

Cryptic human growth hormone gene sequences direct gonadotroph-specific expression in transgenic mice. / Low, M. J.; Goodman, Richard; Ebert, K. M.

In: Molecular Endocrinology, Vol. 3, No. 12, 1989, p. 2028-2033.

Research output: Contribution to journalArticle

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