Cryptic human growth hormone gene sequences direct gonadotroph-specific expression in transgenic mice

Malcolm J. Low, Richard H. Goodman, Karl M. Ebert

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Our laboratory reported previously that chimeric genes encoding either rat somatostatin (SS) or human GH (hGH), but containing the identical mouse metallothionein-l (MT) promoter/enhancer sequences and hGH 3′-flanking sequences, were selectively expressed in the gonadotrophs of transgenic mice. The experiments reported here were designed to identify the DNA sequences responsible for this unexpected cell-specific expression within the anterior pituitary. We produced new transgenic mice expressing fusion genes that tested separately the requirement of the MT or 3′-hGH sequences for gonadotroph expression. A fusion gene that retained the original MT and SS sequences, with a simian virus 40 polyadenylation signal exchanged for the 3′-hGH sequences, no longer directed strong pituitary expression, but was active in the liver. In contrast, a cytomegalovirus promoter/enhancer-SS-hGH fusion gene was expressed at the same high level in the anterior pituitaries of transgenic mice as the originally studied MT-SS-hGH gene. Immunohis-tochemical analysis indicated that pituitary expression of the cytomegalovirus promoter/enhancer-SS-hGH fusion gene was also restricted to gonadotroph cells in adult mice. These studies indicate that sequences within the 3′-flanking region of the hGH gene can direct expression of chimeric genes to pituitary cells that do not normally produce growth hormone.

Original languageEnglish (US)
Pages (from-to)2028-2033
Number of pages6
JournalMolecular Endocrinology
Volume3
Issue number12
DOIs
StatePublished - Dec 1989

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

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