CRF Facilitates Calcium Release from Intracellular Stores in Midbrain Dopamine Neurons

Arthur C. Riegel, John Williams

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Changes in cytosolic calcium are crucial for numerous processes including neuronal plasticity. This study investigates the regulation of cytosolic calcium by corticotropin-releasing factor (CRF) in midbrain dopamine neurons. The results demonstrate that CRF stimulates the release of intracellular calcium from stores through activation of adenylyl cyclase and PKA. Imaging and photolysis experiments showed that the calcium originated from dendrites and required both functional IP3 and ryanodine receptor channels. The elevation in cytosolic calcium potentiated calcium-sensitive potassium channels (sK) activated by action potentials and metabotropic Gq-coupled receptors for glutamate and acetylcholine. This increase in cytosolic calcium activated by postsynaptic Gs-coupled CRF receptors may represent a fundamental mechanism by which stress peptides and hormones can shape Gq-coupled receptor-mediated regulation of neuronal excitability and synaptic plasticity in dopamine neurons.

Original languageEnglish (US)
Pages (from-to)559-570
Number of pages12
JournalNeuron
Volume57
Issue number4
DOIs
StatePublished - Feb 28 2008

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Dopaminergic Neurons
Corticotropin-Releasing Hormone
Mesencephalon
Calcium
Neuronal Plasticity
Corticotropin-Releasing Hormone Receptors
Inositol 1,4,5-Trisphosphate Receptors
Ryanodine Receptor Calcium Release Channel
Peptide Hormones
Photolysis
Potassium Channels
Glutamate Receptors
Cholinergic Receptors
Dendrites
Adenylyl Cyclases
Action Potentials

Keywords

  • MOLNEURO
  • SIGNALING
  • SYSNEURO

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

CRF Facilitates Calcium Release from Intracellular Stores in Midbrain Dopamine Neurons. / Riegel, Arthur C.; Williams, John.

In: Neuron, Vol. 57, No. 4, 28.02.2008, p. 559-570.

Research output: Contribution to journalArticle

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