Creatine kinase-catalyzed reaction rate in the cyanide-poisoned mouse brain

David Holtzman, Martin Offutt, Miles Tsuji, Leo J. Neuringer, Danny Jacobs

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Brain creatine kinase (CK)-catalyzed phosphorus flux from phosphocreatine (PC) to ATP was measured in vivo in young adult mice made reversibly hypoxic by injection of cyanide. Phosphorus spectra and saturation transfer measurements of CK-catalyzed flux were acquired using a high-field (8.45 T) nuclear magnetic resonance (NMR) spectrometer. After low cyanide doses (1-3 mg/kg of body weight), there were no measurable changes in brain pH or in concentrations of PC, the nucleoside triphosphates (including ATP), and Pi. The CK-catalyzed phosphorus flux increased about 75% after the low cyanide dose. Higher doses (4-6 mg/kg) produced a transient 30-40% decrease in PC concentration, doubling of Pi, and a 0.2 unit decrease in pH. The CK-catalyzed phosphorus flux decreased 50-80% after the higher cyanide doses. This decrease in phosphorus flux was present long after reactant concentrations returned to precyanide values. It is proposed that the increase in brain CK-catalyzed phosphorus flux with the lower cyanide doses is due to an increase in ADP concentration. The large, prolonged decrease in CK-catalyzed reaction rate in the moderately poisoned brain may be due to loss of activity of the mitochondrial CK isoform.

Original languageEnglish (US)
Pages (from-to)153-161
Number of pages9
JournalJournal of Cerebral Blood Flow and Metabolism
Volume13
Issue number1
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

Cyanides
Creatine Kinase
Phosphorus
Phosphocreatine
Brain
BB Form Creatine Kinase
Mitochondrial Form Creatine Kinase
Adenosine Triphosphate
Nucleosides
Adenosine Diphosphate
Young Adult
Protein Isoforms
Magnetic Resonance Spectroscopy
Body Weight
Injections

Keywords

  • Brain
  • Creatine kinase
  • Cyanide
  • NMR

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

Cite this

Creatine kinase-catalyzed reaction rate in the cyanide-poisoned mouse brain. / Holtzman, David; Offutt, Martin; Tsuji, Miles; Neuringer, Leo J.; Jacobs, Danny.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 13, No. 1, 01.01.1993, p. 153-161.

Research output: Contribution to journalArticle

Holtzman, David ; Offutt, Martin ; Tsuji, Miles ; Neuringer, Leo J. ; Jacobs, Danny. / Creatine kinase-catalyzed reaction rate in the cyanide-poisoned mouse brain. In: Journal of Cerebral Blood Flow and Metabolism. 1993 ; Vol. 13, No. 1. pp. 153-161.
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