Cortical granule complements in human oocytes undergoing partial zona dissection

Susan E. Lanzendorf; Ralph R. Kazer; Phillip E. Patton; Don P. Wolf

doi:10.1002/mrd.1080310207

Cortical granule complements in human oocytes undergoing partial zona dissection

Susan E. Lanzendorf, Ralph R. Kazer, Phillip E. Patton, Don P. Wolf

Obstetrics and Gynecology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

This study was performed to evaluate the effects of mechanical stimulation and sucrose treatment on the oocyte activation process. Fresh and aged human oocytes were exposed to sucrose and zonae were dissected with microneedles before fixation and quantitative analysis of cortical granules by transmission electron microscopy. Examination of the mean number of cortical granules/analyzed segment revealed no significant differences between control oocytes or oocytes treated with sucrose or sucrose treatment followed by zona dissection. A significant decline in the number of cortical granules/segment was observed for oocytes undergoing prolonged culture after dissection (P < 0.05). Thus, zona dissection and sucrose exposure of freshly aspirated mature human oocytes do not result in classical oocyte activation.

Original language	English (US)
Pages (from-to)	131-134
Number of pages	4
Journal	Molecular Reproduction and Development
Volume	31
Issue number	2
DOIs	https://doi.org/10.1002/mrd.1080310207
State	Published - Feb 1992

Keywords

Activation
PZD
Sucrose treatment

ASJC Scopus subject areas

Genetics
Developmental Biology
Cell Biology

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10.1002/mrd.1080310207

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title = "Cortical granule complements in human oocytes undergoing partial zona dissection",

abstract = "This study was performed to evaluate the effects of mechanical stimulation and sucrose treatment on the oocyte activation process. Fresh and aged human oocytes were exposed to sucrose and zonae were dissected with microneedles before fixation and quantitative analysis of cortical granules by transmission electron microscopy. Examination of the mean number of cortical granules/analyzed segment revealed no significant differences between control oocytes or oocytes treated with sucrose or sucrose treatment followed by zona dissection. A significant decline in the number of cortical granules/segment was observed for oocytes undergoing prolonged culture after dissection (P < 0.05). Thus, zona dissection and sucrose exposure of freshly aspirated mature human oocytes do not result in classical oocyte activation.",

keywords = "Activation, PZD, Sucrose treatment",

author = "Lanzendorf, {Susan E.} and Kazer, {Ralph R.} and Patton, {Phillip E.} and Wolf, {Don P.}",

year = "1992",

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AU - Lanzendorf, Susan E.

AU - Kazer, Ralph R.

AU - Patton, Phillip E.

AU - Wolf, Don P.

PY - 1992/2

Y1 - 1992/2

N2 - This study was performed to evaluate the effects of mechanical stimulation and sucrose treatment on the oocyte activation process. Fresh and aged human oocytes were exposed to sucrose and zonae were dissected with microneedles before fixation and quantitative analysis of cortical granules by transmission electron microscopy. Examination of the mean number of cortical granules/analyzed segment revealed no significant differences between control oocytes or oocytes treated with sucrose or sucrose treatment followed by zona dissection. A significant decline in the number of cortical granules/segment was observed for oocytes undergoing prolonged culture after dissection (P < 0.05). Thus, zona dissection and sucrose exposure of freshly aspirated mature human oocytes do not result in classical oocyte activation.

AB - This study was performed to evaluate the effects of mechanical stimulation and sucrose treatment on the oocyte activation process. Fresh and aged human oocytes were exposed to sucrose and zonae were dissected with microneedles before fixation and quantitative analysis of cortical granules by transmission electron microscopy. Examination of the mean number of cortical granules/analyzed segment revealed no significant differences between control oocytes or oocytes treated with sucrose or sucrose treatment followed by zona dissection. A significant decline in the number of cortical granules/segment was observed for oocytes undergoing prolonged culture after dissection (P < 0.05). Thus, zona dissection and sucrose exposure of freshly aspirated mature human oocytes do not result in classical oocyte activation.

KW - Activation

KW - PZD

KW - Sucrose treatment

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Cortical granule complements in human oocytes undergoing partial zona dissection

Abstract

Keywords

ASJC Scopus subject areas

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