Cortical granule complements in human oocytes undergoing partial zona dissection

Susan E. Lanzendorf, Ralph R. Kazer, Phillip E. Patton, Don P. Wolf

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

This study was performed to evaluate the effects of mechanical stimulation and sucrose treatment on the oocyte activation process. Fresh and aged human oocytes were exposed to sucrose and zonae were dissected with microneedles before fixation and quantitative analysis of cortical granules by transmission electron microscopy. Examination of the mean number of cortical granules/analyzed segment revealed no significant differences between control oocytes or oocytes treated with sucrose or sucrose treatment followed by zona dissection. A significant decline in the number of cortical granules/segment was observed for oocytes undergoing prolonged culture after dissection (P < 0.05). Thus, zona dissection and sucrose exposure of freshly aspirated mature human oocytes do not result in classical oocyte activation.

Original languageEnglish (US)
Pages (from-to)131-134
Number of pages4
JournalMolecular Reproduction and Development
Volume31
Issue number2
DOIs
StatePublished - Feb 1992

Keywords

  • Activation
  • PZD
  • Sucrose treatment

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

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