Correlation of amino acid fucoside labeling patterns with tumorigenicity of mouse mammary epithelial cells

M. R. Steiner, Carolyn (Sue) Richards, J. S. Butel, D. Medina

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The amino acid fucosides of tumorigenic and nontumorigenic mouse mammary gland-derived cells were studied. The cells examined included tumorigenic cell lines derived from mammary carcinomas of the following etiologies: induction by hormone; virus; and chemical carcinogen. Also studied were cells derived from normal mammary glands and several clones of cells, which were derived from a mammary carcinoma but were not demonstrably tumorigenic at lower passage levels after cloning, while they were highly tumorigenic at higher passage levels. Cells were cultured in medium supplemented with radiolabeled fucose and extracted, and extracts were analyzed for the amino acid fucosides. Radiolabeled compounds which comigrated with the amino acid fucosides glucosylfucosylthreonine, fucosylthreonine, and fucosylserine were observed. There was a distinctive difference between the tumorigenic and nontumorigenic cells; the ratio of fucosylthreonine plus fucosylserine to glucosylfucosylthreonine was higher in all tumorigenic cells as compared to the ratio observed for the nontumorigenic cells.

Original languageEnglish (US)
Pages (from-to)2628-2631
Number of pages4
JournalCancer Research
Volume43
Issue number6
StatePublished - 1983
Externally publishedYes

Fingerprint

Breast
Epithelial Cells
Amino Acids
Human Mammary Glands
Breast Neoplasms
Fucose
Carcinogens
Organism Cloning
Cultured Cells
Clone Cells
Hormones
Viruses
Cell Line

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Correlation of amino acid fucoside labeling patterns with tumorigenicity of mouse mammary epithelial cells. / Steiner, M. R.; Richards, Carolyn (Sue); Butel, J. S.; Medina, D.

In: Cancer Research, Vol. 43, No. 6, 1983, p. 2628-2631.

Research output: Contribution to journalArticle

@article{ca66ec4b7eca44179923c8dcbcb9616d,
title = "Correlation of amino acid fucoside labeling patterns with tumorigenicity of mouse mammary epithelial cells",
abstract = "The amino acid fucosides of tumorigenic and nontumorigenic mouse mammary gland-derived cells were studied. The cells examined included tumorigenic cell lines derived from mammary carcinomas of the following etiologies: induction by hormone; virus; and chemical carcinogen. Also studied were cells derived from normal mammary glands and several clones of cells, which were derived from a mammary carcinoma but were not demonstrably tumorigenic at lower passage levels after cloning, while they were highly tumorigenic at higher passage levels. Cells were cultured in medium supplemented with radiolabeled fucose and extracted, and extracts were analyzed for the amino acid fucosides. Radiolabeled compounds which comigrated with the amino acid fucosides glucosylfucosylthreonine, fucosylthreonine, and fucosylserine were observed. There was a distinctive difference between the tumorigenic and nontumorigenic cells; the ratio of fucosylthreonine plus fucosylserine to glucosylfucosylthreonine was higher in all tumorigenic cells as compared to the ratio observed for the nontumorigenic cells.",
author = "Steiner, {M. R.} and Richards, {Carolyn (Sue)} and Butel, {J. S.} and D. Medina",
year = "1983",
language = "English (US)",
volume = "43",
pages = "2628--2631",
journal = "Journal of Cancer Research",
issn = "0099-7013",
publisher = "American Association for Cancer Research Inc.",
number = "6",

}

TY - JOUR

T1 - Correlation of amino acid fucoside labeling patterns with tumorigenicity of mouse mammary epithelial cells

AU - Steiner, M. R.

AU - Richards, Carolyn (Sue)

AU - Butel, J. S.

AU - Medina, D.

PY - 1983

Y1 - 1983

N2 - The amino acid fucosides of tumorigenic and nontumorigenic mouse mammary gland-derived cells were studied. The cells examined included tumorigenic cell lines derived from mammary carcinomas of the following etiologies: induction by hormone; virus; and chemical carcinogen. Also studied were cells derived from normal mammary glands and several clones of cells, which were derived from a mammary carcinoma but were not demonstrably tumorigenic at lower passage levels after cloning, while they were highly tumorigenic at higher passage levels. Cells were cultured in medium supplemented with radiolabeled fucose and extracted, and extracts were analyzed for the amino acid fucosides. Radiolabeled compounds which comigrated with the amino acid fucosides glucosylfucosylthreonine, fucosylthreonine, and fucosylserine were observed. There was a distinctive difference between the tumorigenic and nontumorigenic cells; the ratio of fucosylthreonine plus fucosylserine to glucosylfucosylthreonine was higher in all tumorigenic cells as compared to the ratio observed for the nontumorigenic cells.

AB - The amino acid fucosides of tumorigenic and nontumorigenic mouse mammary gland-derived cells were studied. The cells examined included tumorigenic cell lines derived from mammary carcinomas of the following etiologies: induction by hormone; virus; and chemical carcinogen. Also studied were cells derived from normal mammary glands and several clones of cells, which were derived from a mammary carcinoma but were not demonstrably tumorigenic at lower passage levels after cloning, while they were highly tumorigenic at higher passage levels. Cells were cultured in medium supplemented with radiolabeled fucose and extracted, and extracts were analyzed for the amino acid fucosides. Radiolabeled compounds which comigrated with the amino acid fucosides glucosylfucosylthreonine, fucosylthreonine, and fucosylserine were observed. There was a distinctive difference between the tumorigenic and nontumorigenic cells; the ratio of fucosylthreonine plus fucosylserine to glucosylfucosylthreonine was higher in all tumorigenic cells as compared to the ratio observed for the nontumorigenic cells.

UR - http://www.scopus.com/inward/record.url?scp=0020580650&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020580650&partnerID=8YFLogxK

M3 - Article

C2 - 6406050

AN - SCOPUS:0020580650

VL - 43

SP - 2628

EP - 2631

JO - Journal of Cancer Research

JF - Journal of Cancer Research

SN - 0099-7013

IS - 6

ER -