Correlation of amino acid fucoside labeling patterns with tumorigenicity of mouse mammary epithelial cells

Marion R. Steiner, Carolyn S. Richards, Janet S. Butel, Daniel Medina

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The amino acid fucosides of tumorigenic and nontumorigenic mouse mammary gland-derived cells were studied. The cells examined included tumorigenic cell lines derived from mammary carcinomas of the following etiologies: induction by hormone; virus; and chemical carcinogen. Also studied were cells derived from normal mammary glands and several clones of cells, which were derived from a mammary carcinoma but were not demonstrably tumorigenic at lower passage levels after cloning, while they were highly tumorigenic at higher passage levels. Cells were cultured in medium supplemented with radiolabeled fucose and extracted, and extracts were analyzed for the amino acid fucosides. Radiolabeled compounds which comigrated with the amino acid fucosides glucosylfucosylthreonine, fucosyl-threonine, and fucosylserine were observed. There was a distinctive difference between the tumorigenic and nontumorigenic cells; the ratio of fucosylthreonine plus fucosylserine to gluco-sylfucosylthreonine was higher in all tumorigenic cells as compared to the ratio observed for the nontumorigenic cells.

Original languageEnglish (US)
Pages (from-to)2628-2631
Number of pages4
JournalCancer Research
Volume43
Issue number6
StatePublished - Jun 1 1983

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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