Contamination with E1A-positive wild-type adenovirus accounts for species-specific stimulation of islet cell proliferation by CCK: A cautionary note

Jeremy A. Lavine, Philipp W. Raess, Dawn B. Davis, Mary E. Rabaglia, Brent K. Presley, Mark P. Keller, Margery C. Beinfeld, Alan S. Kopin, Christopher B. Newgard, Alan D. Attie

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Abstract

We have previously reported that adenovirus-mediated expression of preprocholecystokin (CCK) stimulates human and mouse islet cell proliferation. In follow-up studies, we became concerned that the CCK adenovirus might have been contaminated with a wild-type E1A-containing adenovirus. Here we show conclusively that the proliferative effects reported in the original paper in mouse and human islets were not due to CCK expression but rather to a contaminating E1Aexpressing wild-type adenovirus. We also show, however, that CCK expression does have a proliferative effect in rat islets.We hope that our report of the steps taken to detect the wild-type virus contamination, and purification of the contributing viral stocks, will be helpful to other investigators, and that our experience will serve as a cautionary tale for use of adenovirus vectors, especially for studies on cellular replication.

Original languageEnglish (US)
Pages (from-to)464-467
Number of pages4
JournalMolecular Endocrinology
Volume24
Issue number2
DOIs
StatePublished - Feb 1 2010

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ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

Cite this

Lavine, J. A., Raess, P. W., Davis, D. B., Rabaglia, M. E., Presley, B. K., Keller, M. P., Beinfeld, M. C., Kopin, A. S., Newgard, C. B., & Attie, A. D. (2010). Contamination with E1A-positive wild-type adenovirus accounts for species-specific stimulation of islet cell proliferation by CCK: A cautionary note. Molecular Endocrinology, 24(2), 464-467. https://doi.org/10.1210/me.2009-0384