@article{158195cfbe7e44a8872c12006bbdc0ae,
title = "Consensus recommendations for trabecular meshwork cell isolation, characterization and culture",
abstract = "Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods used to validate their identity. Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings.",
keywords = "Aqueous humor dynamics, Conventional outflow, Glaucoma, Intraocular pressure, Ocular hypertension",
author = "Keller, {Kate E.} and Bhattacharya, {Sanjoy K.} and Theresa Borr{\'a}s and Brunner, {Thomas M.} and Sunee Chansangpetch and Clark, {Abbott F.} and Dismuke, {W. Michael} and Yiqin Du and Elliott, {Michael H.} and Ethier, {C. Ross} and Faralli, {Jennifer A.} and Freddo, {Thomas F.} and Rudolf Fuchshofer and Michael Giovingo and Haiyan Gong and Pedro Gonzalez and Alex Huang and Johnstone, {Murray A.} and Kaufman, {Paul L.} and Kelley, {Mary J.} and Knepper, {Paul A.} and Kopczynski, {Casey C.} and Kuchtey, {John G.} and Kuchtey, {Rachel W.} and Kuehn, {Markus H.} and Lieberman, {Raquel L.} and Lin, {Shan C.} and Paloma Liton and Yutao Liu and Elke L{\"u}tjen-Drecoll and Weiming Mao and Marisse Masis-Solano and Fiona McDonnell and McDowell, {Colleen M.} and Overby, {Darryl R.} and Pattabiraman, {Padmanabhan P.} and Raghunathan, {Vijay K.} and Rao, {P. Vasanth} and Rhee, {Douglas J.} and Chowdhury, {Uttio Roy} and Paul Russell and Samples, {John R.} and Donald Schwartz and Stubbs, {Evan B.} and Tamm, {Ernst R.} and Tan, {James C.} and Toris, {Carol B.} and Torrejon, {Karen Y.} and Vranka, {Janice A.} and Wirtz, {Mary K.} and Thomas Yorio and Jie Zhang and Zode, {Gulab S.} and Fautsch, {Michael P.} and Peters, {Donna M.} and Acott, {Ted S.} and Stamer, {W. Daniel}",
note = "Funding Information: The authors thank Griff Samples, Thelma De Souza, Aerie Pharmaceuticals, Bausch and Lomb, Alcon/Novartis, Glaukos and the Western Glaucoma Foundation for continued support of the trabecular meshwork study club; enabling 16 annual scientific gatherings of researchers dedicated to better understanding trabecular meshwork biology and facilitating the assembly of this position paper. Publisher Copyright: {\textcopyright} 2018",
year = "2018",
month = jun,
doi = "10.1016/j.exer.2018.03.001",
language = "English (US)",
volume = "171",
pages = "164--173",
journal = "Experimental Eye Research",
issn = "0014-4835",
publisher = "Academic Press Inc.",
}