Abstract
Confocal laser scanning microscopy (CLSM) is best known and widely used for fluorescence imaging. However, any commercial CLSM can be operated in reflectance mode by setting the microscope's detector to accept the excitation laser wavelength. In this mode, the images are based on the scattering properties of the cell or tissue. This chapter discusses reflectance-mode CLSM (rCLSM), the mechanisms of contrast involved in such images, and examples of how rCLSM is being used to study tissue properties.
| Original language | English (US) |
|---|---|
| Title of host publication | Handbook of Coherent-Domain Optical Methods: Biomedical Diagnostics, Environmental Monitoring, and Materials Science:: Second Edition |
| Publisher | Springer New York |
| Pages | 1157-1171 |
| Number of pages | 15 |
| Volume | 2-2 |
| ISBN (Electronic) | 9781461451761 |
| ISBN (Print) | 9781461451754 |
| DOIs | |
| State | Published - Jan 1 2013 |
| Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)