Confocal laser scanning microscopy using scattering as the contrast mechanism

Steven Jacques

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Confocal laser scanning microscopy (CLSM) is best known and widely used for fluorescence imaging. However, any commercial CLSM can be operated in reflectance mode by setting the microscope's detector to accept the excitation laser wavelength. In this mode, the images are based on the scattering properties of the cell or tissue. This chapter discusses reflectance-mode CLSM (rCLSM), the mechanisms of contrast involved in such images, and examples of how rCLSM is being used to study tissue properties.

Original languageEnglish (US)
Title of host publicationHandbook of Coherent-Domain Optical Methods: Biomedical Diagnostics, Environmental Monitoring, and Materials Science:: Second Edition
PublisherSpringer New York
Pages1157-1171
Number of pages15
Volume2-2
ISBN (Electronic)9781461451761
ISBN (Print)9781461451754
DOIs
StatePublished - Jan 1 2013

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Jacques, S. (2013). Confocal laser scanning microscopy using scattering as the contrast mechanism. In Handbook of Coherent-Domain Optical Methods: Biomedical Diagnostics, Environmental Monitoring, and Materials Science:: Second Edition (Vol. 2-2, pp. 1157-1171). Springer New York. https://doi.org/10.1007/978-1-4614-5176-1_28