Confocal laser scanning microscopy using scattering as the contrast mechanism

Steven L. Jacques

doi:10.1007/978-1-4614-5176-1_28

Confocal laser scanning microscopy using scattering as the contrast mechanism

Steven L. Jacques

Biomedical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Chapter

2 Scopus citations

Abstract

Confocal laser scanning microscopy (CLSM) is best known and widely used for fluorescence imaging. However, any commercial CLSM can be operated in reflectance mode by setting the microscope's detector to accept the excitation laser wavelength. In this mode, the images are based on the scattering properties of the cell or tissue. This chapter discusses reflectance-mode CLSM (rCLSM), the mechanisms of contrast involved in such images, and examples of how rCLSM is being used to study tissue properties.

Original language	English (US)
Title of host publication	Handbook of Coherent-Domain Optical Methods: Biomedical Diagnostics, Environmental Monitoring, and Materials Science:: Second Edition
Publisher	Springer New York
Pages	1157-1171
Number of pages	15
Volume	2-2
ISBN (Electronic)	9781461451761
ISBN (Print)	9781461451754
DOIs	https://doi.org/10.1007/978-1-4614-5176-1_28
State	Published - Jan 1 2013

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

Access to Document

10.1007/978-1-4614-5176-1_28

Cite this

@inbook{b53db82e442645238b29639187ec41e6,

title = "Confocal laser scanning microscopy using scattering as the contrast mechanism",

abstract = "Confocal laser scanning microscopy (CLSM) is best known and widely used for fluorescence imaging. However, any commercial CLSM can be operated in reflectance mode by setting the microscope's detector to accept the excitation laser wavelength. In this mode, the images are based on the scattering properties of the cell or tissue. This chapter discusses reflectance-mode CLSM (rCLSM), the mechanisms of contrast involved in such images, and examples of how rCLSM is being used to study tissue properties.",

author = "Jacques, {Steven L.}",

year = "2013",

month = jan,

day = "1",

doi = "10.1007/978-1-4614-5176-1_28",

language = "English (US)",

isbn = "9781461451754",

volume = "2-2",

pages = "1157--1171",

booktitle = "Handbook of Coherent-Domain Optical Methods: Biomedical Diagnostics, Environmental Monitoring, and Materials Science:: Second Edition",

publisher = "Springer New York",

}

TY - CHAP

T1 - Confocal laser scanning microscopy using scattering as the contrast mechanism

AU - Jacques, Steven L.

PY - 2013/1/1

Y1 - 2013/1/1

N2 - Confocal laser scanning microscopy (CLSM) is best known and widely used for fluorescence imaging. However, any commercial CLSM can be operated in reflectance mode by setting the microscope's detector to accept the excitation laser wavelength. In this mode, the images are based on the scattering properties of the cell or tissue. This chapter discusses reflectance-mode CLSM (rCLSM), the mechanisms of contrast involved in such images, and examples of how rCLSM is being used to study tissue properties.

AB - Confocal laser scanning microscopy (CLSM) is best known and widely used for fluorescence imaging. However, any commercial CLSM can be operated in reflectance mode by setting the microscope's detector to accept the excitation laser wavelength. In this mode, the images are based on the scattering properties of the cell or tissue. This chapter discusses reflectance-mode CLSM (rCLSM), the mechanisms of contrast involved in such images, and examples of how rCLSM is being used to study tissue properties.

UR - http://www.scopus.com/inward/record.url?scp=84956514219&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84956514219&partnerID=8YFLogxK

U2 - 10.1007/978-1-4614-5176-1_28

DO - 10.1007/978-1-4614-5176-1_28

M3 - Chapter

AN - SCOPUS:84956514219

SN - 9781461451754

VL - 2-2

SP - 1157

EP - 1171

BT - Handbook of Coherent-Domain Optical Methods: Biomedical Diagnostics, Environmental Monitoring, and Materials Science:: Second Edition

PB - Springer New York

ER -

Confocal laser scanning microscopy using scattering as the contrast mechanism

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this