Complete sequence of virulence plasmid pJM1 from the marine fish pathogen Vibrio anguillarum strain 775

Manuela Di Lorenzo, Michiel Stork, Marcelo E. Tolmasky, Luis A. Actis, David Farrell, Timothy J. Welch, Lidia M. Crosa, Anne M. Wertheimer, Qian Chen, Patricia Salinas, Lillian Waldbeser, Jorge H. Crosa

Research output: Contribution to journalArticle

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Abstract

The virulence plasmid pJM1 enables the fish pathogen Vibrio anguillarum, a gram-negative polarly flagellated comma-shaped rod bacterium, to cause a highly fatal hemorrhagic septicemic disease in salmonids and other fishes, leading to epizootics throughout the world. The pJM1 plasmid 65,009-nucleotide sequence, with an overall G+C content of 42.6%, revealed genes and open reading frames (ORFs) encoding iron transporters, nonribosomal peptide enzymes, and other proteins essential for the biosynthesis of the siderophore anguibactin. Of the 59 ORFs, approximately 32% were related to iron metabolic functions. The plasmid pJM1 confers on V. anguillarum the ability to take up ferric iron as a complex with anguibactin from a medium in which iron is chelated by transferrin, ethylenediamine-di(o-hydroxyphenyl-acetic acid), or other iron-chelating compounds. The fatDCBA-angRT operon as well as other downstream biosynthetic genes is bracketed by the homologous ISV-A1 and ISV-A2 insertion sequences. Other clusters on the plasmid also show an insertion element-flanked organization, including ORFs homologous to genes involved in the biosynthesis of 2,3-dihydroxybenzoic acid. Homologues of replication and partition genes are also identified on pJM1 adjacent to this region. ORFs with no known function represent approximately 30% of the pJM1 sequence. The insertion sequence elements in the composite transposon-like structures, corroborated by the G+C content of the pJM1 sequence, suggest a modular composition of plasmid pJM1, biased towards acquisition of modules containing genes related to iron metabolic functions. We also show that there is considerable microheterogeneity in pJM1-like plasmids from virulent strains of V. anguillarum isolated from different geographical sources.

Original languageEnglish (US)
Pages (from-to)5822-5830
Number of pages9
JournalJournal of Bacteriology
Volume185
Issue number19
DOIs
StatePublished - Oct 2003

Fingerprint

Vibrio
Virulence
Fishes
Plasmids
Iron
Open Reading Frames
ethylenediamine
Base Composition
Genes
Iron Compounds
Salmonidae
Siderophores
DNA Transposable Elements
Gene Regulatory Networks
Insertional Mutagenesis
Protein Biosynthesis
Operon
Transferrin
varespladib methyl
Bacteria

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Complete sequence of virulence plasmid pJM1 from the marine fish pathogen Vibrio anguillarum strain 775. / Di Lorenzo, Manuela; Stork, Michiel; Tolmasky, Marcelo E.; Actis, Luis A.; Farrell, David; Welch, Timothy J.; Crosa, Lidia M.; Wertheimer, Anne M.; Chen, Qian; Salinas, Patricia; Waldbeser, Lillian; Crosa, Jorge H.

In: Journal of Bacteriology, Vol. 185, No. 19, 10.2003, p. 5822-5830.

Research output: Contribution to journalArticle

Di Lorenzo, M, Stork, M, Tolmasky, ME, Actis, LA, Farrell, D, Welch, TJ, Crosa, LM, Wertheimer, AM, Chen, Q, Salinas, P, Waldbeser, L & Crosa, JH 2003, 'Complete sequence of virulence plasmid pJM1 from the marine fish pathogen Vibrio anguillarum strain 775', Journal of Bacteriology, vol. 185, no. 19, pp. 5822-5830. https://doi.org/10.1128/JB.185.19.5822-5830.2003
Di Lorenzo, Manuela ; Stork, Michiel ; Tolmasky, Marcelo E. ; Actis, Luis A. ; Farrell, David ; Welch, Timothy J. ; Crosa, Lidia M. ; Wertheimer, Anne M. ; Chen, Qian ; Salinas, Patricia ; Waldbeser, Lillian ; Crosa, Jorge H. / Complete sequence of virulence plasmid pJM1 from the marine fish pathogen Vibrio anguillarum strain 775. In: Journal of Bacteriology. 2003 ; Vol. 185, No. 19. pp. 5822-5830.
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AU - Farrell, David

AU - Welch, Timothy J.

AU - Crosa, Lidia M.

AU - Wertheimer, Anne M.

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