The engraftment potential of adult bone marrow and fetal liver hematopoietic progenitor cells was compared following transplantation into 13.5 day gestation NOD/LtSz-scW/J (Ly-5.2) mouse embryos. Donor cells were prepared from mice expressing Ly-5.1 alone (adult bone marrow; C57B1/6), or those co-expressing Ly-5.1 and Ly-5.2 (fetal liver; C57B1/6J × B6.SJL). Cells from fetal liver and bone marrow (6 months of age) were depleted of lineage-positive cells using immunomagnetic beads. Recipient embryos were injected with a maximum of 3ul of a solution containing equal numbers of fetal and adult cells (final concentration 4×105 cells/ul). Four weeks after birth recipients were analyzed. Recipients were considered chimeric if > 0.5% of the cells in peripheral blood (PB) bone marrow (BM). or spleen were of donor origin. Ninety-two percent of recipients were chimeric in the spleen and 77% in the PB whilst only 38% were chimeric in the BM. Similar patterns of engraftment were found in both PB and spleen. Fetal liver-derived cells were found in 100% of the chimeric animals, whereas adult bone marrow engrafted in 60% of chimeric mice. The frequency of donor cells derived donor from fetal liver was 5-10 fold greater than that contributed from adult donor cells. Donor myeloid cells were present in the PB although lymphoid (T- and B) cells predominated in both PB and spleen. The bone marrow of engrafted recipients typically showed low levels of donor cells between 0.5 and 1%. These results clearly demonstrate that fetal liver-derived hematopoietic progenitor cells have a higher engraftment potential on a per cell basis than adult BM-derived progenitor cells. Ongoing secondary transplant studies will define the frequency of transplantable hematopoietic stem cells derived from fetal live and adult BM.
|Original language||English (US)|
|Number of pages||1|
|State||Published - Dec 1 1997|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology
- Cancer Research