Comparison of gentamicin distribution in the inner ear following administration via the endolymphatic sac or round window

Yiliang Zhang, Ru Zhang, Chunfu Dai, Peter Steyger, Yu Y. Yongfu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objective/Hypothesis: The distribution of gentamicin in the inner ear via the endolymphatic sac (ES) or round window (RW) routes was investigated. Study Design: Experimental study. Methods: A fluorescent gentamicin-Texas Red conjugate (GTTR) was adopted to visualize the gentamicin. Adult guinea pigs were treated with GTTR applied to the ES or RW, the animals were allowed to recover for 3 days, then confocal microscopy was used to observe and quantify GTTR distributions in cochlear and vestibular sensory epithelium. Results: When GTTR was applied via the ES, strong GTTR labeling was observed in the vestibule while little GTTR was detected in the cochlea (P <.0001). However, distinct GTTR fluorescence was observed in the cochlea and vestibule following RW application (P = .7967). There was less GTTR labeling in cochlea via ES application than through RW administration (P <.0001). Conclusions: ES drug application may be preferable for the treatment of intractable Meniere's disease.

Original languageEnglish (US)
Pages (from-to)2054-2060
Number of pages7
JournalLaryngoscope
Volume120
Issue number10
DOIs
StatePublished - Oct 2010

Fingerprint

Endolymphatic Sac
Inner Ear
Gentamicins
Cochlea
Meniere Disease
Texas red
Confocal Microscopy
Guinea Pigs
Research Design
Epithelium
Fluorescence

Keywords

  • Endolymphatic sac
  • Gentamicin
  • Meniere's disease
  • Round window

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Comparison of gentamicin distribution in the inner ear following administration via the endolymphatic sac or round window. / Zhang, Yiliang; Zhang, Ru; Dai, Chunfu; Steyger, Peter; Yongfu, Yu Y.

In: Laryngoscope, Vol. 120, No. 10, 10.2010, p. 2054-2060.

Research output: Contribution to journalArticle

Zhang, Yiliang ; Zhang, Ru ; Dai, Chunfu ; Steyger, Peter ; Yongfu, Yu Y. / Comparison of gentamicin distribution in the inner ear following administration via the endolymphatic sac or round window. In: Laryngoscope. 2010 ; Vol. 120, No. 10. pp. 2054-2060.
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