Cloning of the cDNA and gene for a human D2 dopamine receptor

D. K. Grandy, M. A. Marchionni, H. Makam, R. E. Stofko, M. Alfano, L. Frothingham, J. B. Fischer, K. J. Burke-Howie, J. R. Bunzow, A. C. Server, O. Civelli

    Research output: Contribution to journalArticle

    456 Scopus citations

    Abstract

    A clone encoding a human D2 dopamine receptor was isolated from a pituitary cDNA library and sequenced. The deduced protein sequence is 96% identical with that of the cloned rat receptor with one major difference: the human receptor contains an additional 29 amino acids in its putative third cytoplasmic loop. Southern blotting demonstrated the presence of only one human D2 receptor gene. Two overlapping phage containing the gene were isolated and characterized. DNA sequence analysis of these clones showed that the coding sequence is interrupted by six introns and that the additional amino acids present in the human pituitary receptor are encoded by a single exon of 87 base pairs. The involvement of this sequence in alternative splicing and its biological significance are discussed.

    Original languageEnglish (US)
    Pages (from-to)9762-9766
    Number of pages5
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume86
    Issue number24
    DOIs
    StatePublished - 1989

    Keywords

    • DRD2
    • Schizophrenia
    • alternative splicing
    • introns
    • receptor evolution

    ASJC Scopus subject areas

    • General

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  • Cite this

    Grandy, D. K., Marchionni, M. A., Makam, H., Stofko, R. E., Alfano, M., Frothingham, L., Fischer, J. B., Burke-Howie, K. J., Bunzow, J. R., Server, A. C., & Civelli, O. (1989). Cloning of the cDNA and gene for a human D2 dopamine receptor. Proceedings of the National Academy of Sciences of the United States of America, 86(24), 9762-9766. https://doi.org/10.1073/pnas.86.24.9762