Cloning, mapping, and in vivo localization of a human member of the PKCI-1 protein family (PRKCNH1)

Pius M. Brzoska, Hongying Chen, Nikki A. Levin, Wen Lin Kuo, Colin Collins, Karen K. Fu, Joe W. Gray, Michael F. Christman

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

We report here the complete cDNA sequence, genomic mapping, and immunolocalization of the first human member of the protein kinase C inhibitor (PKCI-1) gene family. The predicted human protein (hPKCI-1) is 96% identical to bovine and 53% identical to maize members, indicating the great evolutionary conservation of this protein family. The hPKCI-1 gene (HGMV- approved symbol PRKCNH1) maps to human chromosome 5q31.2 by fluorescence in situ hybridization. Indirect immunofluorescence shows that hPKCI-1 localizes to cytoskeletal structures in the cytoplasm of a human fibroblast cell line and is largely excluded from the nucleus. The cytoplasmic localization of hPKCI 1 is consistent with a postulated role in mediating a membrane-derived signal in response to ionizing radiation.

Original languageEnglish (US)
Pages (from-to)151-156
Number of pages6
JournalGenomics
Volume36
Issue number1
DOIs
StatePublished - Aug 15 1996

ASJC Scopus subject areas

  • Genetics

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    Brzoska, P. M., Chen, H., Levin, N. A., Kuo, W. L., Collins, C., Fu, K. K., Gray, J. W., & Christman, M. F. (1996). Cloning, mapping, and in vivo localization of a human member of the PKCI-1 protein family (PRKCNH1). Genomics, 36(1), 151-156. https://doi.org/10.1006/geno.1996.0435