Cloning, mapping, and in vivo localization of a human member of the PKCI-1 protein family (PRKCNH1)

Pius M. Brzoska; Hongying Chen; Nikki A. Levin; Wen Lin Kuo; Colin Collins; Karen K. Fu; Joe W. Gray; Michael F. Christman

doi:10.1006/geno.1996.0435

Cloning, mapping, and in vivo localization of a human member of the PKCI-1 protein family (PRKCNH1)

Pius M. Brzoska, Hongying Chen, Nikki A. Levin, Wen Lin Kuo, Colin Collins, Karen K. Fu, Joe W. Gray, Michael F. Christman

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

We report here the complete cDNA sequence, genomic mapping, and immunolocalization of the first human member of the protein kinase C inhibitor (PKCI-1) gene family. The predicted human protein (hPKCI-1) is 96% identical to bovine and 53% identical to maize members, indicating the great evolutionary conservation of this protein family. The hPKCI-1 gene (HGMV- approved symbol PRKCNH1) maps to human chromosome 5q31.2 by fluorescence in situ hybridization. Indirect immunofluorescence shows that hPKCI-1 localizes to cytoskeletal structures in the cytoplasm of a human fibroblast cell line and is largely excluded from the nucleus. The cytoplasmic localization of hPKCI 1 is consistent with a postulated role in mediating a membrane-derived signal in response to ionizing radiation.

Original language	English (US)
Pages (from-to)	151-156
Number of pages	6
Journal	Genomics
Volume	36
Issue number	1
DOIs	https://doi.org/10.1006/geno.1996.0435
State	Published - Aug 15 1996
Externally published	Yes

ASJC Scopus subject areas

Genetics

Access to Document

10.1006/geno.1996.0435

Cite this

@article{4dee79bac7ef4357b37c40a2b5bf021d,

title = "Cloning, mapping, and in vivo localization of a human member of the PKCI-1 protein family (PRKCNH1)",

abstract = "We report here the complete cDNA sequence, genomic mapping, and immunolocalization of the first human member of the protein kinase C inhibitor (PKCI-1) gene family. The predicted human protein (hPKCI-1) is 96% identical to bovine and 53% identical to maize members, indicating the great evolutionary conservation of this protein family. The hPKCI-1 gene (HGMV- approved symbol PRKCNH1) maps to human chromosome 5q31.2 by fluorescence in situ hybridization. Indirect immunofluorescence shows that hPKCI-1 localizes to cytoskeletal structures in the cytoplasm of a human fibroblast cell line and is largely excluded from the nucleus. The cytoplasmic localization of hPKCI 1 is consistent with a postulated role in mediating a membrane-derived signal in response to ionizing radiation.",

author = "Brzoska, {Pius M.} and Hongying Chen and Levin, {Nikki A.} and Kuo, {Wen Lin} and Colin Collins and Fu, {Karen K.} and Gray, {Joe W.} and Christman, {Michael F.}",

note = "Funding Information: We thank M. P. Walsh for providing us with polyclonal antibodies against bovine PKCI-1 and Pragati Bakshi for help in preparing this manuscript. This work was supported by grants to M.F.C. from the AT Children's Project and the American Cancer Society.",

year = "1996",

month = aug,

day = "15",

doi = "10.1006/geno.1996.0435",

language = "English (US)",

volume = "36",

pages = "151--156",

journal = "Genomics",

issn = "0888-7543",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Cloning, mapping, and in vivo localization of a human member of the PKCI-1 protein family (PRKCNH1)

AU - Brzoska, Pius M.

AU - Chen, Hongying

AU - Levin, Nikki A.

AU - Kuo, Wen Lin

AU - Collins, Colin

AU - Fu, Karen K.

AU - Gray, Joe W.

AU - Christman, Michael F.

N1 - Funding Information: We thank M. P. Walsh for providing us with polyclonal antibodies against bovine PKCI-1 and Pragati Bakshi for help in preparing this manuscript. This work was supported by grants to M.F.C. from the AT Children's Project and the American Cancer Society.

PY - 1996/8/15

Y1 - 1996/8/15

N2 - We report here the complete cDNA sequence, genomic mapping, and immunolocalization of the first human member of the protein kinase C inhibitor (PKCI-1) gene family. The predicted human protein (hPKCI-1) is 96% identical to bovine and 53% identical to maize members, indicating the great evolutionary conservation of this protein family. The hPKCI-1 gene (HGMV- approved symbol PRKCNH1) maps to human chromosome 5q31.2 by fluorescence in situ hybridization. Indirect immunofluorescence shows that hPKCI-1 localizes to cytoskeletal structures in the cytoplasm of a human fibroblast cell line and is largely excluded from the nucleus. The cytoplasmic localization of hPKCI 1 is consistent with a postulated role in mediating a membrane-derived signal in response to ionizing radiation.

AB - We report here the complete cDNA sequence, genomic mapping, and immunolocalization of the first human member of the protein kinase C inhibitor (PKCI-1) gene family. The predicted human protein (hPKCI-1) is 96% identical to bovine and 53% identical to maize members, indicating the great evolutionary conservation of this protein family. The hPKCI-1 gene (HGMV- approved symbol PRKCNH1) maps to human chromosome 5q31.2 by fluorescence in situ hybridization. Indirect immunofluorescence shows that hPKCI-1 localizes to cytoskeletal structures in the cytoplasm of a human fibroblast cell line and is largely excluded from the nucleus. The cytoplasmic localization of hPKCI 1 is consistent with a postulated role in mediating a membrane-derived signal in response to ionizing radiation.

UR - http://www.scopus.com/inward/record.url?scp=0030586910&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030586910&partnerID=8YFLogxK

U2 - 10.1006/geno.1996.0435

DO - 10.1006/geno.1996.0435

M3 - Article

C2 - 8812426

AN - SCOPUS:0030586910

SN - 0888-7543

VL - 36

SP - 151

EP - 156

JO - Genomics

JF - Genomics

IS - 1

ER -

Cloning, mapping, and in vivo localization of a human member of the PKCI-1 protein family (PRKCNH1)

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this