Cloning, mapping, and in vivo localization of a human member of the PKCI-1 protein family (PRKCNH1)

Pius M. Brzoska, Hongying Chen, Nikki A. Levin, Wen Lin Kuo, Colin Collins, Karen K. Fu, Joe Gray, Michael F. Christman

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We report here the complete cDNA sequence, genomic mapping, and immunolocalization of the first human member of the protein kinase C inhibitor (PKCI-1) gene family. The predicted human protein (hPKCI-1) is 96% identical to bovine and 53% identical to maize members, indicating the great evolutionary conservation of this protein family. The hPKCI-1 gene (HGMV- approved symbol PRKCNH1) maps to human chromosome 5q31.2 by fluorescence in situ hybridization. Indirect immunofluorescence shows that hPKCI-1 localizes to cytoskeletal structures in the cytoplasm of a human fibroblast cell line and is largely excluded from the nucleus. The cytoplasmic localization of hPKCI 1 is consistent with a postulated role in mediating a membrane-derived signal in response to ionizing radiation.

Original languageEnglish (US)
Pages (from-to)151-156
Number of pages6
JournalGenomics
Volume36
Issue number1
DOIs
StatePublished - Aug 15 1996
Externally publishedYes

Fingerprint

Organism Cloning
Protein C Inhibitor
Human Chromosomes
Protein Kinase Inhibitors
Indirect Fluorescent Antibody Technique
Ionizing Radiation
Fluorescence In Situ Hybridization
Protein Kinase C
Genes
Zea mays
Cytoplasm
Proteins
Complementary DNA
Fibroblasts
Cell Line
Membranes
human HINT1 protein

ASJC Scopus subject areas

  • Genetics

Cite this

Brzoska, P. M., Chen, H., Levin, N. A., Kuo, W. L., Collins, C., Fu, K. K., ... Christman, M. F. (1996). Cloning, mapping, and in vivo localization of a human member of the PKCI-1 protein family (PRKCNH1). Genomics, 36(1), 151-156. https://doi.org/10.1006/geno.1996.0435

Cloning, mapping, and in vivo localization of a human member of the PKCI-1 protein family (PRKCNH1). / Brzoska, Pius M.; Chen, Hongying; Levin, Nikki A.; Kuo, Wen Lin; Collins, Colin; Fu, Karen K.; Gray, Joe; Christman, Michael F.

In: Genomics, Vol. 36, No. 1, 15.08.1996, p. 151-156.

Research output: Contribution to journalArticle

Brzoska, PM, Chen, H, Levin, NA, Kuo, WL, Collins, C, Fu, KK, Gray, J & Christman, MF 1996, 'Cloning, mapping, and in vivo localization of a human member of the PKCI-1 protein family (PRKCNH1)', Genomics, vol. 36, no. 1, pp. 151-156. https://doi.org/10.1006/geno.1996.0435
Brzoska, Pius M. ; Chen, Hongying ; Levin, Nikki A. ; Kuo, Wen Lin ; Collins, Colin ; Fu, Karen K. ; Gray, Joe ; Christman, Michael F. / Cloning, mapping, and in vivo localization of a human member of the PKCI-1 protein family (PRKCNH1). In: Genomics. 1996 ; Vol. 36, No. 1. pp. 151-156.
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