TY - JOUR
T1 - Cloning and Sequence Determination of a Complementary DNA Related to Human Liver Microsomal Cytochrome P-450 S-Mephenytoin 4-Hydroxylase
AU - Umbenhauer, Diane R.
AU - Martin, Martha V.
AU - Lloyd, R. Stephen
AU - Guengerich, F. Peter
PY - 1987
Y1 - 1987
N2 - A Cdna sequence related to the human cytochrome P-450 responsible for S-mephenytoin 4-hydroxylation (P-450mp) has been isolated from a human liver bacteriophage ygt11 library with antibodies specific for P-450mp. The total length of the cDNA is 2.5 kilobases (kb), of which there is a 1.6-kb EcoRI fragment coding for all but five amino acids corresponding to the N-terminus of the protein and including a small noncoding region at the 3’ end. This 1.6-kb fragment has been sequenced and used as a probe to analyze human genomic DNA and liver RNA. The sequence shows extensive sequence similarity with that of rabbit liver cytochrome P-450 progesterone 21-hydroxylase [Tukey, R. H., Okino, S., Barnes, H., Griffin, K. J., & Johnson, E. F. (1985) J. Biol. Chem. 260, 13347–13354], and this cDNA, like the rabbit clone, appears to be part of a multigene family. At least two liver mRNA species, 2.2 kb and 3.5 kb, hybridize to the cDNA sequence. The cloning of this gene should aid in analyzing the molecular basis for the genetic polymorphism of S-mephenytoin 4-hydroxylation reported in humans.
AB - A Cdna sequence related to the human cytochrome P-450 responsible for S-mephenytoin 4-hydroxylation (P-450mp) has been isolated from a human liver bacteriophage ygt11 library with antibodies specific for P-450mp. The total length of the cDNA is 2.5 kilobases (kb), of which there is a 1.6-kb EcoRI fragment coding for all but five amino acids corresponding to the N-terminus of the protein and including a small noncoding region at the 3’ end. This 1.6-kb fragment has been sequenced and used as a probe to analyze human genomic DNA and liver RNA. The sequence shows extensive sequence similarity with that of rabbit liver cytochrome P-450 progesterone 21-hydroxylase [Tukey, R. H., Okino, S., Barnes, H., Griffin, K. J., & Johnson, E. F. (1985) J. Biol. Chem. 260, 13347–13354], and this cDNA, like the rabbit clone, appears to be part of a multigene family. At least two liver mRNA species, 2.2 kb and 3.5 kb, hybridize to the cDNA sequence. The cloning of this gene should aid in analyzing the molecular basis for the genetic polymorphism of S-mephenytoin 4-hydroxylation reported in humans.
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U2 - 10.1021/bi00378a016
DO - 10.1021/bi00378a016
M3 - Article
C2 - 3032244
AN - SCOPUS:0023121619
SN - 0006-2960
VL - 26
SP - 1094
EP - 1099
JO - Biochemistry
JF - Biochemistry
IS - 4
ER -