Cloning and developmental regulation of α1 acid glycoprotein in swine

R. T. Stone, R. A. Maurer

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

A cDNA clone of porcine alpha1 acid glycoprotein (α1AGP) has been isolated and sequenced. Sequence homologies between porcine, human, and rat indicate that porcine α1AGP is similar in structure to the rat and human proteins. RNA blots from days 40, 60, 80, and 110 fetal, newborn, and adult livers showed that α1AGP mRNA is relatively abundant throughout fetal development, particularly at the later stages and in the newborn; there is a rapid decline in abundance following birth. From birth to 3 days of age, there is a three‐ to four‐fold decline in abundance, and α1AGP mRNA is approximately 100 times less abundant in the adult liver than in that of perinatal pigs. Southern blots showed that α1AGP is probably a single‐copy gene. The isolation of a cloned cDNA for porcine α1AGP provides a tool to investigate the molecular mechanisms involved in the developmental regulation of the gene and to correlate changes in gene expression during development with fetal growth and well being.

Original languageEnglish (US)
Pages (from-to)295-304
Number of pages10
JournalDevelopmental Genetics
Volume8
Issue number4
DOIs
StatePublished - 1987

Keywords

  • cDNA
  • fetal pig
  • sequence

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

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