Cloning and characterization of the proximal promoter region of the rat insulin-like growth factor I (IGF-I) receptor gene

Haim Werner, Bethel Stannard, Mark A. Bach, Derek LeRoith, Charles T. Roberts

Research output: Contribution to journalArticlepeer-review

66 Scopus citations

Abstract

We have isolated genomic clones that contain the promoter region of the rat IGF-I receptor gene. A unique transcriptional start site was suggested by the results of primer extension and RNase protection assays, which also defined a 940-base 5′-untranslated region. Despite the single start site, the proximal 415 base pairs of 5′-flanking region were devoid of TATA or CCAAT elements. The region surrounding the start site was, however, similar to a recently described "initiator" sequence that can direct specific transcription initiation in the absence of a TATA element. The 5′-flanking region was GC-rich and contained several possible SP1 sites, but also included potential ETF and AP-2 binding sites. The rat IGF-I receptor gene promoter region appears to have some sequences similar to both "housekeeping" and highly regulated promoters and may be an example of an intermediary class of regulatory region.

Original languageEnglish (US)
Pages (from-to)1021-1027
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume169
Issue number3
DOIs
StatePublished - Jun 29 1990
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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