Cloning and characterization of a calcium-activated chloride channel in rat uterus

Junfang Song, Xiaojuan Zhang, Zhi Qi, Gang Sun, Shaopeng Chi, Zhaohui Zhu, Jiangong Ren, Zhaojun Qiu, Ka Liu, Leslie Myatt, Runlin Z. Ma

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

In a search for genes involved in regulation of uterine contractility, we cloned a novel calcium-activated chloride channel gene, named rat Clca4, from pregnant rat uterus. The gene shares approximately 83% and 70% nucleotide homology with mouse Clca6 and human CLCA4, respectively, and was expressed primarily in rat uterus. The transcripts were upregulated at Gestational Day 22 (prior to parturition), implying a functional involvement in parturition. Western blot analysis showed that rat CLCA4 protein was present in uterus, lung, and heart, but not in any other tissues examined. Confocal microscopy revealed that rat CLCA4 is localized in cell membrane and could not be removed by alkaline or PBS washing. Transient transfection of rat CLCA4-enhanced green fluorescent protein in Chinese hamster ovary cells resulted in production of characteristic Cl- currents that could be activated by Ca 2+ and ionomycin but inhibited by niflumic acid, a CLCA- channel blocker. The identification and characterization of rat Clca4 help decipher the contribution of Ca 2+-activated Cl- conductance in myometrial contractility.

Original languageEnglish (US)
Pages (from-to)788-794
Number of pages7
JournalBiology of reproduction
Volume80
Issue number4
DOIs
StatePublished - Apr 1 2009

Keywords

  • Calcium
  • Calcium-activated chloride channel
  • Gene regulation
  • Myometrium
  • Parturition
  • Pregnancy
  • Uterus

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology

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  • Cite this

    Song, J., Zhang, X., Qi, Z., Sun, G., Chi, S., Zhu, Z., Ren, J., Qiu, Z., Liu, K., Myatt, L., & Ma, R. Z. (2009). Cloning and characterization of a calcium-activated chloride channel in rat uterus. Biology of reproduction, 80(4), 788-794. https://doi.org/10.1095/biolreprod.108.071258