Abstract
We investigated the effects of new inositol 1,4,5-trisphosphate analogues on the release of Ca2+ from isolated vacuoles of Neurospora crassa. Tri-O-butyryl-inositol 1,4,5-trisphosphate and a set of cis,cis-cyclohexane 1,3,5-triol bis-(CHT-P2) and trisphosphates (CHT-P3) gave an increase in free Ca2+ as measured directly with fura-2, a Ca2+-chelator. However, inositol 1,4-bisphosphate, 6-O-palmitoyl-inositol 4,5-bisphosphate and trans-cyclohexane 1,2-diol bisphosphate (trans CHD-P2) did not induce Ca2+-release. These results suggest that the 1,5-bisphosphate position in inositol 1,4,5-trisphosphate (Ins 1,4,5-P3) is the only essential arrangement for receptor binding to vacuoles of Neurospora crassa. The structures of these analogues are discussed on the basis of a general concept for the design of new Ins 1,4,5-P3 analogogues.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1319-1327 |
| Number of pages | 9 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 166 |
| Issue number | 3 |
| DOIs | |
| State | Published - Feb 14 1990 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
cis,cis-Cyclohexane 1,3,5-triol polyphosphates release calcium from Neurospora crassa via an unspecific ins 1,4,5-P3 receptor. / Schultz, Carsten; Gebauer, G.; Metschies, T.; Rensing, L.; Jastorff, B.
In: Biochemical and Biophysical Research Communications, Vol. 166, No. 3, 14.02.1990, p. 1319-1327.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - cis,cis-Cyclohexane 1,3,5-triol polyphosphates release calcium from Neurospora crassa via an unspecific ins 1,4,5-P3 receptor
AU - Schultz, Carsten
AU - Gebauer, G.
AU - Metschies, T.
AU - Rensing, L.
AU - Jastorff, B.
PY - 1990/2/14
Y1 - 1990/2/14
N2 - We investigated the effects of new inositol 1,4,5-trisphosphate analogues on the release of Ca2+ from isolated vacuoles of Neurospora crassa. Tri-O-butyryl-inositol 1,4,5-trisphosphate and a set of cis,cis-cyclohexane 1,3,5-triol bis-(CHT-P2) and trisphosphates (CHT-P3) gave an increase in free Ca2+ as measured directly with fura-2, a Ca2+-chelator. However, inositol 1,4-bisphosphate, 6-O-palmitoyl-inositol 4,5-bisphosphate and trans-cyclohexane 1,2-diol bisphosphate (trans CHD-P2) did not induce Ca2+-release. These results suggest that the 1,5-bisphosphate position in inositol 1,4,5-trisphosphate (Ins 1,4,5-P3) is the only essential arrangement for receptor binding to vacuoles of Neurospora crassa. The structures of these analogues are discussed on the basis of a general concept for the design of new Ins 1,4,5-P3 analogogues.
AB - We investigated the effects of new inositol 1,4,5-trisphosphate analogues on the release of Ca2+ from isolated vacuoles of Neurospora crassa. Tri-O-butyryl-inositol 1,4,5-trisphosphate and a set of cis,cis-cyclohexane 1,3,5-triol bis-(CHT-P2) and trisphosphates (CHT-P3) gave an increase in free Ca2+ as measured directly with fura-2, a Ca2+-chelator. However, inositol 1,4-bisphosphate, 6-O-palmitoyl-inositol 4,5-bisphosphate and trans-cyclohexane 1,2-diol bisphosphate (trans CHD-P2) did not induce Ca2+-release. These results suggest that the 1,5-bisphosphate position in inositol 1,4,5-trisphosphate (Ins 1,4,5-P3) is the only essential arrangement for receptor binding to vacuoles of Neurospora crassa. The structures of these analogues are discussed on the basis of a general concept for the design of new Ins 1,4,5-P3 analogogues.
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UR - http://www.scopus.com/inward/citedby.url?scp=0025190174&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(90)91010-P
DO - 10.1016/0006-291X(90)91010-P
M3 - Article
C2 - 2154977
AN - SCOPUS:0025190174
VL - 166
SP - 1319
EP - 1327
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -