cis,cis-Cyclohexane 1,3,5-triol polyphosphates release calcium from Neurospora crassa via an unspecific ins 1,4,5-P3 receptor

C. Schultz, G. Gebauer, T. Metschies, L. Rensing, B. Jastorff

Research output: Contribution to journalArticle

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Abstract

We investigated the effects of new inositol 1,4,5-trisphosphate analogues on the release of Ca2+ from isolated vacuoles of Neurospora crassa. Tri-O-butyryl-inositol 1,4,5-trisphosphate and a set of cis,cis-cyclohexane 1,3,5-triol bis-(CHT-P2) and trisphosphates (CHT-P3) gave an increase in free Ca2+ as measured directly with fura-2, a Ca2+-chelator. However, inositol 1,4-bisphosphate, 6-O-palmitoyl-inositol 4,5-bisphosphate and trans-cyclohexane 1,2-diol bisphosphate (trans CHD-P2) did not induce Ca2+-release. These results suggest that the 1,5-bisphosphate position in inositol 1,4,5-trisphosphate (Ins 1,4,5-P3) is the only essential arrangement for receptor binding to vacuoles of Neurospora crassa. The structures of these analogues are discussed on the basis of a general concept for the design of new Ins 1,4,5-P3 analogogues.

Original languageEnglish (US)
Pages (from-to)1319-1327
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume166
Issue number3
DOIs
StatePublished - Feb 14 1990

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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