cis,cis-Cyclohexane 1,3,5-triol polyphosphates release calcium from Neurospora crassa via an unspecific ins 1,4,5-P3 receptor

C. Schultz; G. Gebauer; T. Metschies; L. Rensing; B. Jastorff

doi:10.1016/0006-291X(90)91010-P

cis,cis-Cyclohexane 1,3,5-triol polyphosphates release calcium from Neurospora crassa via an unspecific ins 1,4,5-P₃ receptor

C. Schultz, G. Gebauer, T. Metschies, L. Rensing, B. Jastorff

Physiology & Pharmacology

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

We investigated the effects of new inositol 1,4,5-trisphosphate analogues on the release of Ca²⁺ from isolated vacuoles of Neurospora crassa. Tri-O-butyryl-inositol 1,4,5-trisphosphate and a set of cis,cis-cyclohexane 1,3,5-triol bis-(CHT-P₂) and trisphosphates (CHT-P₃) gave an increase in free Ca²⁺ as measured directly with fura-2, a Ca²⁺-chelator. However, inositol 1,4-bisphosphate, 6-O-palmitoyl-inositol 4,5-bisphosphate and trans-cyclohexane 1,2-diol bisphosphate (trans CHD-P₂) did not induce Ca²⁺-release. These results suggest that the 1,5-bisphosphate position in inositol 1,4,5-trisphosphate (Ins 1,4,5-P₃) is the only essential arrangement for receptor binding to vacuoles of Neurospora crassa. The structures of these analogues are discussed on the basis of a general concept for the design of new Ins 1,4,5-P₃ analogogues.

Original language	English (US)
Pages (from-to)	1319-1327
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	166
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(90)91010-P
State	Published - Feb 14 1990

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "cis,cis-Cyclohexane 1,3,5-triol polyphosphates release calcium from Neurospora crassa via an unspecific ins 1,4,5-P3 receptor",

abstract = "We investigated the effects of new inositol 1,4,5-trisphosphate analogues on the release of Ca2+ from isolated vacuoles of Neurospora crassa. Tri-O-butyryl-inositol 1,4,5-trisphosphate and a set of cis,cis-cyclohexane 1,3,5-triol bis-(CHT-P2) and trisphosphates (CHT-P3) gave an increase in free Ca2+ as measured directly with fura-2, a Ca2+-chelator. However, inositol 1,4-bisphosphate, 6-O-palmitoyl-inositol 4,5-bisphosphate and trans-cyclohexane 1,2-diol bisphosphate (trans CHD-P2) did not induce Ca2+-release. These results suggest that the 1,5-bisphosphate position in inositol 1,4,5-trisphosphate (Ins 1,4,5-P3) is the only essential arrangement for receptor binding to vacuoles of Neurospora crassa. The structures of these analogues are discussed on the basis of a general concept for the design of new Ins 1,4,5-P3 analogogues.",

author = "C. Schultz and G. Gebauer and T. Metschies and L. Rensing and B. Jastorff",

year = "1990",

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T1 - cis,cis-Cyclohexane 1,3,5-triol polyphosphates release calcium from Neurospora crassa via an unspecific ins 1,4,5-P3 receptor

AU - Schultz, C.

AU - Gebauer, G.

AU - Metschies, T.

AU - Rensing, L.

AU - Jastorff, B.

PY - 1990/2/14

Y1 - 1990/2/14

N2 - We investigated the effects of new inositol 1,4,5-trisphosphate analogues on the release of Ca2+ from isolated vacuoles of Neurospora crassa. Tri-O-butyryl-inositol 1,4,5-trisphosphate and a set of cis,cis-cyclohexane 1,3,5-triol bis-(CHT-P2) and trisphosphates (CHT-P3) gave an increase in free Ca2+ as measured directly with fura-2, a Ca2+-chelator. However, inositol 1,4-bisphosphate, 6-O-palmitoyl-inositol 4,5-bisphosphate and trans-cyclohexane 1,2-diol bisphosphate (trans CHD-P2) did not induce Ca2+-release. These results suggest that the 1,5-bisphosphate position in inositol 1,4,5-trisphosphate (Ins 1,4,5-P3) is the only essential arrangement for receptor binding to vacuoles of Neurospora crassa. The structures of these analogues are discussed on the basis of a general concept for the design of new Ins 1,4,5-P3 analogogues.

AB - We investigated the effects of new inositol 1,4,5-trisphosphate analogues on the release of Ca2+ from isolated vacuoles of Neurospora crassa. Tri-O-butyryl-inositol 1,4,5-trisphosphate and a set of cis,cis-cyclohexane 1,3,5-triol bis-(CHT-P2) and trisphosphates (CHT-P3) gave an increase in free Ca2+ as measured directly with fura-2, a Ca2+-chelator. However, inositol 1,4-bisphosphate, 6-O-palmitoyl-inositol 4,5-bisphosphate and trans-cyclohexane 1,2-diol bisphosphate (trans CHD-P2) did not induce Ca2+-release. These results suggest that the 1,5-bisphosphate position in inositol 1,4,5-trisphosphate (Ins 1,4,5-P3) is the only essential arrangement for receptor binding to vacuoles of Neurospora crassa. The structures of these analogues are discussed on the basis of a general concept for the design of new Ins 1,4,5-P3 analogogues.

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