Chronic prenatal cocaine treatment down-regulates μ-opioid receptor mRNA expression in the brain of fetal Rhesus Macaque

Lin Chai; Martha A. Bosch; Josephine M. Moore; Oline K. Rønnekleiv

doi:10.1016/S0304-3940(98)01016-7

Chronic prenatal cocaine treatment down-regulates μ-opioid receptor mRNA expression in the brain of fetal Rhesus Macaque

Lin Chai, Martha A. Bosch, Josephine M. Moore, Oline K. Rønnekleiv

Physiology & Pharmacology

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Ribonuclease protection assays (RPA) were performed to quantify μ- opioid receptor mRNA expression in specific brain regions of day 70 Rhesus Macaque fetuses that were exposed to cocaine (3 mg/kg) or saline from days 22-70 of gestation. The content of μ-receptor mRNA was high in the diencephalon and moderate in the mesencephalon. In contrast, μ-receptor mRNA was lightly expressed in areas such as the frontal codex, striatum and the temporal lobe. The content of μ-opioid receptor mRNA was significantly higher in the diencephalon than in other brain regions (P < 0.001; n = 4). Cocaine exposure significantly decreased the expression of μ-receptor mRNA in the fetal diencephalon (P < 0.05; n = 4 in each group). Our data would indicate that prolonged gestational cocaine exposure causes μ-opioid receptor mRNA down-regulation in specific brain regions of the fetus.

Original language	English (US)
Pages (from-to)	45-48
Number of pages	4
Journal	Neuroscience Letters
Volume	261
Issue number	1-2
DOIs	https://doi.org/10.1016/S0304-3940(98)01016-7
State	Published - Feb 12 1999

Keywords

Cocaine
Monkey fetus
Ribonuclease protection assay
μ-Opioid receptors

ASJC Scopus subject areas

General Neuroscience

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10.1016/S0304-3940(98)01016-7

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title = "Chronic prenatal cocaine treatment down-regulates μ-opioid receptor mRNA expression in the brain of fetal Rhesus Macaque",

abstract = "Ribonuclease protection assays (RPA) were performed to quantify μ- opioid receptor mRNA expression in specific brain regions of day 70 Rhesus Macaque fetuses that were exposed to cocaine (3 mg/kg) or saline from days 22-70 of gestation. The content of μ-receptor mRNA was high in the diencephalon and moderate in the mesencephalon. In contrast, μ-receptor mRNA was lightly expressed in areas such as the frontal codex, striatum and the temporal lobe. The content of μ-opioid receptor mRNA was significantly higher in the diencephalon than in other brain regions (P < 0.001; n = 4). Cocaine exposure significantly decreased the expression of μ-receptor mRNA in the fetal diencephalon (P < 0.05; n = 4 in each group). Our data would indicate that prolonged gestational cocaine exposure causes μ-opioid receptor mRNA down-regulation in specific brain regions of the fetus.",

keywords = "Cocaine, Monkey fetus, Ribonuclease protection assay, μ-Opioid receptors",

author = "Lin Chai and Bosch, {Martha A.} and Moore, {Josephine M.} and R{\o}nnekleiv, {Oline K.}",

note = "Funding Information: This work was supported by U.S. Public Health Service Grant DA07165 and Animal Resources Branch Grant RR-00163. L.C. and J.M.M. were supported in part by Public Health Service Grant T32 HD01733. ",

year = "1999",

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doi = "10.1016/S0304-3940(98)01016-7",

language = "English (US)",

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T1 - Chronic prenatal cocaine treatment down-regulates μ-opioid receptor mRNA expression in the brain of fetal Rhesus Macaque

AU - Chai, Lin

AU - Bosch, Martha A.

AU - Moore, Josephine M.

AU - Rønnekleiv, Oline K.

N1 - Funding Information: This work was supported by U.S. Public Health Service Grant DA07165 and Animal Resources Branch Grant RR-00163. L.C. and J.M.M. were supported in part by Public Health Service Grant T32 HD01733.

PY - 1999/2/12

Y1 - 1999/2/12

N2 - Ribonuclease protection assays (RPA) were performed to quantify μ- opioid receptor mRNA expression in specific brain regions of day 70 Rhesus Macaque fetuses that were exposed to cocaine (3 mg/kg) or saline from days 22-70 of gestation. The content of μ-receptor mRNA was high in the diencephalon and moderate in the mesencephalon. In contrast, μ-receptor mRNA was lightly expressed in areas such as the frontal codex, striatum and the temporal lobe. The content of μ-opioid receptor mRNA was significantly higher in the diencephalon than in other brain regions (P < 0.001; n = 4). Cocaine exposure significantly decreased the expression of μ-receptor mRNA in the fetal diencephalon (P < 0.05; n = 4 in each group). Our data would indicate that prolonged gestational cocaine exposure causes μ-opioid receptor mRNA down-regulation in specific brain regions of the fetus.

AB - Ribonuclease protection assays (RPA) were performed to quantify μ- opioid receptor mRNA expression in specific brain regions of day 70 Rhesus Macaque fetuses that were exposed to cocaine (3 mg/kg) or saline from days 22-70 of gestation. The content of μ-receptor mRNA was high in the diencephalon and moderate in the mesencephalon. In contrast, μ-receptor mRNA was lightly expressed in areas such as the frontal codex, striatum and the temporal lobe. The content of μ-opioid receptor mRNA was significantly higher in the diencephalon than in other brain regions (P < 0.001; n = 4). Cocaine exposure significantly decreased the expression of μ-receptor mRNA in the fetal diencephalon (P < 0.05; n = 4 in each group). Our data would indicate that prolonged gestational cocaine exposure causes μ-opioid receptor mRNA down-regulation in specific brain regions of the fetus.

KW - Cocaine

KW - Monkey fetus

KW - Ribonuclease protection assay

KW - μ-Opioid receptors

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Chronic prenatal cocaine treatment down-regulates μ-opioid receptor mRNA expression in the brain of fetal Rhesus Macaque

Abstract

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