Chronic prenatal cocaine treatment down-regulates μ-opioid receptor mRNA expression in the brain of fetal Rhesus Macaque

Lin Chai, Martha A. Bosch, Josephine M. Moore, Oline Ronnekleiv

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Ribonuclease protection assays (RPA) were performed to quantify μ- opioid receptor mRNA expression in specific brain regions of day 70 Rhesus Macaque fetuses that were exposed to cocaine (3 mg/kg) or saline from days 22-70 of gestation. The content of μ-receptor mRNA was high in the diencephalon and moderate in the mesencephalon. In contrast, μ-receptor mRNA was lightly expressed in areas such as the frontal codex, striatum and the temporal lobe. The content of μ-opioid receptor mRNA was significantly higher in the diencephalon than in other brain regions (P <0.001; n = 4). Cocaine exposure significantly decreased the expression of μ-receptor mRNA in the fetal diencephalon (P <0.05; n = 4 in each group). Our data would indicate that prolonged gestational cocaine exposure causes μ-opioid receptor mRNA down-regulation in specific brain regions of the fetus.

Original languageEnglish (US)
Pages (from-to)45-48
Number of pages4
JournalNeuroscience Letters
Volume261
Issue number1-2
DOIs
StatePublished - Feb 12 1999

Fingerprint

Opioid Receptors
Macaca mulatta
Cocaine
Down-Regulation
Diencephalon
Messenger RNA
Brain
Fetus
Temporal Lobe
Ribonucleases
Mesencephalon
Pregnancy

Keywords

  • μ-Opioid receptors
  • Cocaine
  • Monkey fetus
  • Ribonuclease protection assay

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Chronic prenatal cocaine treatment down-regulates μ-opioid receptor mRNA expression in the brain of fetal Rhesus Macaque. / Chai, Lin; Bosch, Martha A.; Moore, Josephine M.; Ronnekleiv, Oline.

In: Neuroscience Letters, Vol. 261, No. 1-2, 12.02.1999, p. 45-48.

Research output: Contribution to journalArticle

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AB - Ribonuclease protection assays (RPA) were performed to quantify μ- opioid receptor mRNA expression in specific brain regions of day 70 Rhesus Macaque fetuses that were exposed to cocaine (3 mg/kg) or saline from days 22-70 of gestation. The content of μ-receptor mRNA was high in the diencephalon and moderate in the mesencephalon. In contrast, μ-receptor mRNA was lightly expressed in areas such as the frontal codex, striatum and the temporal lobe. The content of μ-opioid receptor mRNA was significantly higher in the diencephalon than in other brain regions (P <0.001; n = 4). Cocaine exposure significantly decreased the expression of μ-receptor mRNA in the fetal diencephalon (P <0.05; n = 4 in each group). Our data would indicate that prolonged gestational cocaine exposure causes μ-opioid receptor mRNA down-regulation in specific brain regions of the fetus.

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