Chromosome transfer in mature oocytes.

Masahito Tachibana, Michelle Sparman, Shoukhrat Mitalipov

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

To demonstrate step-by-step micromanipulation procedures required for transfer of spindle-chromosomal complexes between mature oocytes. Video presentation of reproductive biology study. In vitro fertilization and embryo manipulation laboratory. Rhesus (Macaca mulatta) primates. Transplantation of the genetic material between mammalian oocytes offers many opportunities to study various aspects of nuclear-cytoplasmic interactions during oogenesis, fertilization and embryo development. We demonstrate the feasibility of isolation and transfer of chromosomes between mature metaphase II (MII) primate oocyte. After fertilization, manipulated oocytes were capable of producing healthy offspring or embryonic stem cells. In this video, we show micromanipulation procedures required for isolation and transfer of spindle-chromosomal complexes between rhesus MII oocytes. In brief, the spindle is visualized using a polarized microscope and extracted into a membrane enclosed karyoplast. Karyoplasts are then reintroduced into an enucleated recipient oocyte (cytoplast, derived from an another female) by karyoplast-cytoplast membrane fusion. Newly reconstructed oocytes consist of nuclear genetic material from one female and cytoplasmic components, including mitochondria and mitochondrial DNA from another. This video demonstrates the protocol developed for primate oocytes that successfully allowed of isolation and transfer of chromosomes between mature metaphase II (MII) oocytes. Potential clinical applications include mitochondrial gene replacement therapy to prevent transmission of mtDNA mutations and treatment of infertility caused by cytoplasmic defects in oocytes. Video is available at http://fertstertforum.com/2012974tachibana/.

Original languageEnglish (US)
JournalFertility and Sterility
Volume97
Issue number5
StatePublished - May 2012
Externally publishedYes

Fingerprint

Oocytes
Chromosomes
Metaphase
Micromanipulation
Primates
Mitochondrial DNA
Fertilization
Oogenesis
Membrane Fusion
Mitochondrial Genes
Fertilization in Vitro
Embryonic Stem Cells
Macaca mulatta
Genetic Therapy
Infertility
Genes
Embryonic Development
Mitochondria
Embryonic Structures
Transplantation

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Reproductive Medicine

Cite this

Chromosome transfer in mature oocytes. / Tachibana, Masahito; Sparman, Michelle; Mitalipov, Shoukhrat.

In: Fertility and Sterility, Vol. 97, No. 5, 05.2012.

Research output: Contribution to journalArticle

Tachibana, M, Sparman, M & Mitalipov, S 2012, 'Chromosome transfer in mature oocytes.', Fertility and Sterility, vol. 97, no. 5.
Tachibana, Masahito ; Sparman, Michelle ; Mitalipov, Shoukhrat. / Chromosome transfer in mature oocytes. In: Fertility and Sterility. 2012 ; Vol. 97, No. 5.
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