Choline transporter-like protein 4 (CTL4) links to non-neuronal acetylcholine synthesis

Pingfang Song, Stephen S. Rekow, Corey Ayne Singleton, Harmanjatinder S. Sekhon, Gregory Dissen, Minerva Zhou, Barbara Campling, Jon Lindstrom, Eliot Spindel

    Research output: Contribution to journalArticle

    27 Citations (Scopus)

    Abstract

    Synthesis of acetylcholine (ACh) by non-neuronal cells is now well established and plays diverse physiologic roles. In neurons, the Na +-dependent, high affinity choline transporter (CHT1) is absolutely required for ACh synthesis. In contrast, some non-neuronal cells synthesize ACh in the absence of CHT1 indicating a fundamental difference in ACh synthesis compared to neurons. The aim of this study was to identify choline transporters, other than CHT1, that play a role in non-neuronal ACh synthesis. ACh synthesis was studied in lung and colon cancer cell lines focusing on the choline transporter-like proteins, a five gene family choline-transporter like protein (CTL)1-5. Supporting a role for CTLs in choline transport in lung cancer cells, choline transport was Na+-independent and CTL1-5 were expressed in all cells examined. CTL1, 2, and 5 were expressed at highest levels and knockdown of CTL1, 2, and 5 decreased choline transport in H82 lung cancer cells. Knockdowns of CTL1, 2, 3, and 5 had no effect on ACh synthesis in H82 cells. In contrast, knockdown of CTL4 significantly decreased ACh secretion by both lung and colon cancer cells. Conversely, increasing expression of CTL4 increased ACh secretion. These results indicate that CTL4 mediates ACh synthesis in non-neuronal cell lines and presents a mechanism to target non-neuronal ACh synthesis without affecting neuronal ACh synthesis. It is now well established that many non-neuronal cell types synthesize and secrete acetylcholine that acts as an autocrine or paracrine hormone. Although there are many similarities between neuronal and non-neuronal acetylcholine synthesis, there is a difference in choline transport for acetylcholine synthesis. Neurons must use the high affinity choline transporter CHT1, but many non-neuronal cells synthesize acetylcholine without CHT1. In this article, we show that non-neuronal cells utilize the choline transporter-like protein-4 (CTL4) for ACh synthesis.

    Original languageEnglish (US)
    Pages (from-to)451-461
    Number of pages11
    JournalJournal of Neurochemistry
    Volume126
    Issue number4
    DOIs
    StatePublished - Aug 2013

    Fingerprint

    Acetylcholine
    Proteins
    Choline
    Cells
    Lung Neoplasms
    Neurons
    choline transporter
    link protein
    Colonic Neoplasms
    Cell Line

    Keywords

    • acetylcholine
    • choline
    • choline transporter
    • choline transporter-like protein
    • CTL
    • lung cancer

    ASJC Scopus subject areas

    • Biochemistry
    • Cellular and Molecular Neuroscience

    Cite this

    Choline transporter-like protein 4 (CTL4) links to non-neuronal acetylcholine synthesis. / Song, Pingfang; Rekow, Stephen S.; Singleton, Corey Ayne; Sekhon, Harmanjatinder S.; Dissen, Gregory; Zhou, Minerva; Campling, Barbara; Lindstrom, Jon; Spindel, Eliot.

    In: Journal of Neurochemistry, Vol. 126, No. 4, 08.2013, p. 451-461.

    Research output: Contribution to journalArticle

    Song, P, Rekow, SS, Singleton, CA, Sekhon, HS, Dissen, G, Zhou, M, Campling, B, Lindstrom, J & Spindel, E 2013, 'Choline transporter-like protein 4 (CTL4) links to non-neuronal acetylcholine synthesis', Journal of Neurochemistry, vol. 126, no. 4, pp. 451-461. https://doi.org/10.1111/jnc.12298
    Song, Pingfang ; Rekow, Stephen S. ; Singleton, Corey Ayne ; Sekhon, Harmanjatinder S. ; Dissen, Gregory ; Zhou, Minerva ; Campling, Barbara ; Lindstrom, Jon ; Spindel, Eliot. / Choline transporter-like protein 4 (CTL4) links to non-neuronal acetylcholine synthesis. In: Journal of Neurochemistry. 2013 ; Vol. 126, No. 4. pp. 451-461.
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    AU - Sekhon, Harmanjatinder S.

    AU - Dissen, Gregory

    AU - Zhou, Minerva

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