Chemoenzymatic enrichment of phosphotyrosine-containing peptides

Shuwei Li, Dexing Zeng

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

(Chemical Equation Presented) Change the Tyr: A tyrosinase-assisted chemoenzymatic method was used to modify and enrich phosphotyrosine-containing peptides from a peptide mixture. This approach could be used to study protein tyrosine phosphorylation, and represents a promising alternative to immunoaffinity purification by using antiphosphotyrosine antibodies.

Original languageEnglish (US)
Pages (from-to)4751-4753
Number of pages3
JournalAngewandte Chemie - International Edition
Volume46
Issue number25
DOIs
StatePublished - Jul 4 2007
Externally publishedYes

Fingerprint

Phosphotyrosine
Peptides
Phosphorylation
Monophenol Monooxygenase
Antibodies
Purification
Tyrosine
Proteins

Keywords

  • Biotin
  • Mass spectrometry
  • Proteomics
  • Tyrosinases
  • Tyrosine phosphorylation

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

Cite this

Chemoenzymatic enrichment of phosphotyrosine-containing peptides. / Li, Shuwei; Zeng, Dexing.

In: Angewandte Chemie - International Edition, Vol. 46, No. 25, 04.07.2007, p. 4751-4753.

Research output: Contribution to journalArticle

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