Characterization of the androgen receptor from a syrian hamster ductus deferens tumor cell line (DDT1)

James S. Norris, Peter Kohler

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The hamster ductus deferens cloned tumor cell line (DDT1) contains a complex steroid receptor protein that binds 3H-labeled 5α-dihydrotestosterone. Diethylaminoethyl (DEAE) chromatography of cytosol from these cells yields two major receptor peaks of activity. Identification of this steroid binding protein as a cytoplasmic receptor was confirmed by salt dissociation on sucrose gradients, stability of the hormone-receptor complex at 0°C, and the retention patterns on phosphocellulose and DEAE cellulose. Multiple forms of the receptor exist in a single homogeneous cell type. The data support the theory that steroid hormones bind to a cytoplasmic protein receptor composed of dissimilar subunits as the initial step in steroid hormone action.

Original languageEnglish (US)
Pages (from-to)898-900
Number of pages3
JournalScience
Volume192
Issue number4242
StatePublished - 1976
Externally publishedYes

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Vas Deferens
Mesocricetus
Androgen Receptors
Tumor Cell Line
Steroids
Hormones
Cytoplasmic and Nuclear Receptors
Dihydrotestosterone
Steroid Receptors
Cellulose
Cricetinae
Cytosol
Sucrose
Chromatography
Carrier Proteins
Proteins
Salts

ASJC Scopus subject areas

  • General

Cite this

Characterization of the androgen receptor from a syrian hamster ductus deferens tumor cell line (DDT1). / Norris, James S.; Kohler, Peter.

In: Science, Vol. 192, No. 4242, 1976, p. 898-900.

Research output: Contribution to journalArticle

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