Characterization of insulin-like growth factor binding proteins of cultured rat astroglial and neuronal cells

Ian Ocrant, Hung Pham, Youngman Oh, Ron G. Rosenfeld

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

Insulin-like growth factor binding proteins produced by cultured rat neurons, astrocytes, and rat cell lines BRL-3A and B104 were compared to binding proteins found in rat serum, using affinity labeling, deglycosylation, and Western ligand blotting studies. Each source elaborated an unique pattern of heterogeneous binding proteins. Some of the binding proteins from different sources behaved similarly in each experimental system suggesting that subsets of these binding proteins may be structurally related. In particular, our data suggest that cultured astrocytes and neurons make the major binding protein produced by BRL-3A cells.

Original languageEnglish (US)
Pages (from-to)1316-1322
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume159
Issue number3
DOIs
StatePublished - Mar 31 1989

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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