Characterization of human uveal melanoma cells by phosphorus 31 nuclear magnetic resonance spectroscopy

N. H. Kolodny, Daniel Albert, J. Epstein, M. Ruzzo, M. Sprengnether

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We performed experiments to determine the potential usefulness of nuclear magnetic resonance spectra in the diagnosis and follow-up of ocular melanoma. High-resolution phosphorus 31 nuclear magnetic resonance spectra at 109.3 MHz were obtained for human uveal melanoma, Greene hamster melanoma, and normal human diploid fibroblast cells. Phosphate metabolites were identified and their concentrations were shown to vary among the different cell lines. Uveal melanoma cells contain unusually high concentrations of the phospholipid metabolite phosphorylcholine and the phosphodiesters glycerol 3-phosphoryl choline and glycerol 3-phosphoryl ethanolamine. Baseline data are thus provided for studies of the effect of various treatment modalities on uveal melanoma. These initial results suggest that the data provided by high-resolution phosphorus 31 nuclear magnetic resonance spectra can provide useful diagnostic and follow-up data with respect to ocular melanoma.

Original languageEnglish (US)
Pages (from-to)38-44
Number of pages7
JournalAmerican Journal of Ophthalmology
Volume100
Issue number1
DOIs
StatePublished - Jan 1 1985
Externally publishedYes

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Phosphorus
Melanoma
Magnetic Resonance Spectroscopy
Glycerol
Ethanolamine
Phosphorylcholine
Choline
Diploidy
Cricetinae
Phospholipids
Fibroblasts
Phosphates
Cell Line
Uveal melanoma

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Characterization of human uveal melanoma cells by phosphorus 31 nuclear magnetic resonance spectroscopy. / Kolodny, N. H.; Albert, Daniel; Epstein, J.; Ruzzo, M.; Sprengnether, M.

In: American Journal of Ophthalmology, Vol. 100, No. 1, 01.01.1985, p. 38-44.

Research output: Contribution to journalArticle

Kolodny, N. H. ; Albert, Daniel ; Epstein, J. ; Ruzzo, M. ; Sprengnether, M. / Characterization of human uveal melanoma cells by phosphorus 31 nuclear magnetic resonance spectroscopy. In: American Journal of Ophthalmology. 1985 ; Vol. 100, No. 1. pp. 38-44.
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