Characterization of cytokines present in middle ear effusions

Robert F. Yellon, Gerald Leonard, Phillip Marucha, Robert Craven, Robert J. Carpenter, William B. Lehmann, Joseph A. Burleson, Donald L. Kreutzer

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

Retention of inflammatory mediators and cells in the middle ear cleft during chronic otitis media with effusion (COME), results in ongoing inflammation with the potential for pathologic changes and hearing loss. Cytokines are glycoproteins produced by macrophages and other cells. Activities of cytokines include fever production, osteoclast, fibroblast, phagocyte and cytotoxic cell activation, regulation of antibody formation, and inhibition of cartilage, bone and endothelial cell growth. Using enzyme-linked immunospecific assays we measured levels of six cytokines in middle ear effusions (MEE) from children with COME. Significant levels of four cytokines: in- terleukin-l-beta (>50 pg/ml), interleukin-2 (>300 pg/ml), tumor necrosis factor-alpha (>40 pg/ml), and gamma-interferon (>6.25 pg/ml) were found in 51%, 54%, 63%, and 19% of MEE, respectively. In contrast, levels of a fifth cytokine, granulocyte-macrophage colony-stimulating factor, and a sixth cytokine, inter- leukin-4, were undetectable. Age was observed to have a significant effect on the levels of specific cytokines. Interleukin-1 (IL-1) correlated inversely (P

Original languageEnglish (US)
Pages (from-to)165-169
Number of pages5
JournalLaryngoscope
Volume101
Issue number2
StatePublished - 1991
Externally publishedYes

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Otitis Media with Effusion
Cytokines
Interleukins
Osteoclasts
Middle Ear
Granulocyte-Macrophage Colony-Stimulating Factor
Phagocytes
Interleukin-1
Hearing Loss
Interleukin-4
Interferon-gamma
Cartilage
Antibody Formation
Interleukin-2
Glycoproteins
Fever
Endothelial Cells
Tumor Necrosis Factor-alpha
Fibroblasts
Macrophages

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Yellon, R. F., Leonard, G., Marucha, P., Craven, R., Carpenter, R. J., Lehmann, W. B., ... Kreutzer, D. L. (1991). Characterization of cytokines present in middle ear effusions. Laryngoscope, 101(2), 165-169.

Characterization of cytokines present in middle ear effusions. / Yellon, Robert F.; Leonard, Gerald; Marucha, Phillip; Craven, Robert; Carpenter, Robert J.; Lehmann, William B.; Burleson, Joseph A.; Kreutzer, Donald L.

In: Laryngoscope, Vol. 101, No. 2, 1991, p. 165-169.

Research output: Contribution to journalArticle

Yellon, RF, Leonard, G, Marucha, P, Craven, R, Carpenter, RJ, Lehmann, WB, Burleson, JA & Kreutzer, DL 1991, 'Characterization of cytokines present in middle ear effusions', Laryngoscope, vol. 101, no. 2, pp. 165-169.
Yellon RF, Leonard G, Marucha P, Craven R, Carpenter RJ, Lehmann WB et al. Characterization of cytokines present in middle ear effusions. Laryngoscope. 1991;101(2):165-169.
Yellon, Robert F. ; Leonard, Gerald ; Marucha, Phillip ; Craven, Robert ; Carpenter, Robert J. ; Lehmann, William B. ; Burleson, Joseph A. ; Kreutzer, Donald L. / Characterization of cytokines present in middle ear effusions. In: Laryngoscope. 1991 ; Vol. 101, No. 2. pp. 165-169.
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