Characterization of anti-competitor activities produced by oral bacteria.

Fengxia Qi, Jens Kreth

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Most bacteria in nature exist in multispecies communities known as biofilms. In the natural habitat where resources (nutrient, space, etc.) are usually limited, individual species must compete or collaborate with other neighboring species in order to perpetuate in the multispecies community. The human oral cavity is colonized by >700 microbial species known as the indigenous microflora. This indigenous flora normally maintains an ecological balance through antagonistic as well as mutualistic interspecies interactions. However, environmental perturbation may disrupt this balance, leading to overgrowth of pathogenic species, which could in turn initiate diseases such as dental caries (tooth decay) and periodontitis (gum disease). Understanding the mechanisms of diversity maintenance may help development of novel approaches to manage these "polymicrobial diseases." In this chapter, we will focus on a well-characterized form of biochemical warfare: bacteriocins produced by Streptococcus mutans, a primary dental caries pathogen, and H(2)O(2) produced by Streptococcus sanguinis, an oral commensal. We will describe detailed methodologies on the competition assay, isolation, purification, and characterization of bacteriocins.

Original languageEnglish (US)
Pages (from-to)151-166
Number of pages16
JournalMethods in molecular biology (Clifton, N.J.)
Volume666
StatePublished - Dec 1 2010
Externally publishedYes

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Bacteriocins
Dental Caries
Bacteria
Streptococcus mutans
Deciduous Tooth
Periodontitis
Gingiva
Biofilms
Streptococcus
Ecosystem
Mouth
Tooth
Maintenance
Food
Natural Resources
Warfare

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Characterization of anti-competitor activities produced by oral bacteria. / Qi, Fengxia; Kreth, Jens.

In: Methods in molecular biology (Clifton, N.J.), Vol. 666, 01.12.2010, p. 151-166.

Research output: Contribution to journalArticle

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