Characterization of an autophosphorylation-dependent multifunctional protein kinase from liver.

S. D. Yang, S. Y. Chang, Thomas Soderling

Research output: Contribution to journalArticle

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Abstract

A cyclic nucleotide- and Ca2+-independent protein kinase has been identified and purified from pig liver to apparent homogeneity. This independent protein kinase is basically inactive but can be activated by a 4-min incubation with 0.25 mM ATP and 2 mM Mg2+. This ATP X Mg-mediated activation appears to involve an intramolecular autophosphorylation as it is independent of kinase concentration. Phosphoamino acid analysis further indicates that this intramolecular autophosphorylation/activation process is predominantly on a serine residue. The nonphosphorylated, inactive form of the kinase is extremely trypsin-labile, whereas the phosphorylated, active kinase is more resistant to trypsin, suggesting a conformational change during the activation process. Autophosphorylation/activation of the kinase is enhanced 2-fold by heparin (0.4 unit/ml) and blocked by phosphatidylserine (0.4 mg/ml). Partial dephosphorylation of the phosphorylated kinase is associated with a time-dependent decrease in the enzyme activity. This autophosphorylation-dependent protein kinase phosphorylates glycogen synthase (Km = 8 microM) at sites 2 and 3, resulting in inactivation of glycogen synthase. The results indicate that this independent kinase may represent a previously undiscovered liver multifunctional protein kinase which can be regulated by reversible phosphorylation.

Original languageEnglish (US)
Pages (from-to)9421-9427
Number of pages7
JournalJournal of Biological Chemistry
Volume262
Issue number19
StatePublished - Jul 5 1987
Externally publishedYes

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Liver
Phosphotransferases
Protein Kinases
Chemical activation
Glycogen Synthase
Trypsin
Adenosine Triphosphate
Phosphoamino Acids
Phosphorylation
Cyclic Nucleotides
Phosphatidylserines
Enzyme activity
Serine
Heparin
autophosphorylation-dependent multifunctional protein kinase
Swine
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Characterization of an autophosphorylation-dependent multifunctional protein kinase from liver. / Yang, S. D.; Chang, S. Y.; Soderling, Thomas.

In: Journal of Biological Chemistry, Vol. 262, No. 19, 05.07.1987, p. 9421-9427.

Research output: Contribution to journalArticle

Yang, S. D. ; Chang, S. Y. ; Soderling, Thomas. / Characterization of an autophosphorylation-dependent multifunctional protein kinase from liver. In: Journal of Biological Chemistry. 1987 ; Vol. 262, No. 19. pp. 9421-9427.
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