Characteristics of acetylcholine-activated channels of innervated and chronically denervated skeletal muscles

Charles Allen, Edson X. Albuquerque

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Characteristics of the ACh-activated channels before and after denervation of the frog interosseal muscle were studied using the patch clamp technique. Acetylcholine sensitivity was increased on extrajunctional portions of the muscle 7, 42, and 73 days after sectioning of the sciatic nerve. Nonjunctional regions of the innervated muscle appeared to contain one type of ACh channels having a conductance of 28 pS and a mean channel lifetime of 3.8 ms at -90 mV. The denervated muscles contained two classes of channels with conductance of 18 and 28 pS which were present as early as 7 days postdenervation and remained for 93 days. The channel open times of the innervated muscles increased with membrane hyperolarization. The open times of the channels present at 42 days postdenervation showed longer lifetimes than those of innervated muscles and were 10.8 ms and 9.6 ms at -90 mV. These channels also showed less voltage dependence than the control fibers.

Original languageEnglish (US)
Pages (from-to)532-545
Number of pages14
JournalExperimental Neurology
Volume91
Issue number3
DOIs
StatePublished - 1986
Externally publishedYes

Fingerprint

Acetylcholine
Skeletal Muscle
Muscles
Patch-Clamp Techniques
Denervation
Sciatic Nerve
Anura
Membranes

ASJC Scopus subject areas

  • Neuroscience(all)
  • Neurology

Cite this

Characteristics of acetylcholine-activated channels of innervated and chronically denervated skeletal muscles. / Allen, Charles; Albuquerque, Edson X.

In: Experimental Neurology, Vol. 91, No. 3, 1986, p. 532-545.

Research output: Contribution to journalArticle

@article{a83f2d3060bd4394888c8dc2cdd2bd54,
title = "Characteristics of acetylcholine-activated channels of innervated and chronically denervated skeletal muscles",
abstract = "Characteristics of the ACh-activated channels before and after denervation of the frog interosseal muscle were studied using the patch clamp technique. Acetylcholine sensitivity was increased on extrajunctional portions of the muscle 7, 42, and 73 days after sectioning of the sciatic nerve. Nonjunctional regions of the innervated muscle appeared to contain one type of ACh channels having a conductance of 28 pS and a mean channel lifetime of 3.8 ms at -90 mV. The denervated muscles contained two classes of channels with conductance of 18 and 28 pS which were present as early as 7 days postdenervation and remained for 93 days. The channel open times of the innervated muscles increased with membrane hyperolarization. The open times of the channels present at 42 days postdenervation showed longer lifetimes than those of innervated muscles and were 10.8 ms and 9.6 ms at -90 mV. These channels also showed less voltage dependence than the control fibers.",
author = "Charles Allen and Albuquerque, {Edson X.}",
year = "1986",
doi = "10.1016/0014-4886(86)90050-6",
language = "English (US)",
volume = "91",
pages = "532--545",
journal = "Experimental Neurology",
issn = "0014-4886",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Characteristics of acetylcholine-activated channels of innervated and chronically denervated skeletal muscles

AU - Allen, Charles

AU - Albuquerque, Edson X.

PY - 1986

Y1 - 1986

N2 - Characteristics of the ACh-activated channels before and after denervation of the frog interosseal muscle were studied using the patch clamp technique. Acetylcholine sensitivity was increased on extrajunctional portions of the muscle 7, 42, and 73 days after sectioning of the sciatic nerve. Nonjunctional regions of the innervated muscle appeared to contain one type of ACh channels having a conductance of 28 pS and a mean channel lifetime of 3.8 ms at -90 mV. The denervated muscles contained two classes of channels with conductance of 18 and 28 pS which were present as early as 7 days postdenervation and remained for 93 days. The channel open times of the innervated muscles increased with membrane hyperolarization. The open times of the channels present at 42 days postdenervation showed longer lifetimes than those of innervated muscles and were 10.8 ms and 9.6 ms at -90 mV. These channels also showed less voltage dependence than the control fibers.

AB - Characteristics of the ACh-activated channels before and after denervation of the frog interosseal muscle were studied using the patch clamp technique. Acetylcholine sensitivity was increased on extrajunctional portions of the muscle 7, 42, and 73 days after sectioning of the sciatic nerve. Nonjunctional regions of the innervated muscle appeared to contain one type of ACh channels having a conductance of 28 pS and a mean channel lifetime of 3.8 ms at -90 mV. The denervated muscles contained two classes of channels with conductance of 18 and 28 pS which were present as early as 7 days postdenervation and remained for 93 days. The channel open times of the innervated muscles increased with membrane hyperolarization. The open times of the channels present at 42 days postdenervation showed longer lifetimes than those of innervated muscles and were 10.8 ms and 9.6 ms at -90 mV. These channels also showed less voltage dependence than the control fibers.

UR - http://www.scopus.com/inward/record.url?scp=0022653189&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022653189&partnerID=8YFLogxK

U2 - 10.1016/0014-4886(86)90050-6

DO - 10.1016/0014-4886(86)90050-6

M3 - Article

VL - 91

SP - 532

EP - 545

JO - Experimental Neurology

JF - Experimental Neurology

SN - 0014-4886

IS - 3

ER -