Characterisation of atrial natriuretic peptide receptors in bovine ventricular sarcolemma

Shirley McCartney, James F. Aiton, Gordon Cramb

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Analysis of [125I]-ANP binding data in an isolated bovine ventricular sarcolemmal membrane fraction revealed a single high affinity binding site (Kd approximately 5 × 10-11 M). The ring deleted ANP analogue des [QSGLG]-ANP (4-23)-NH2 bound with a 1000-fold lower affinity indicating the absence of C-type receptors in this preparation. ANP stimulated guanylate cyclase activity by up to 2-fold with half-maximal activation at approximately 10-9 M. Crosslinking [125I]-ANP to its receptor with disuccinimidyl suberate (DSS) revealed two radiolabelled bands of 120 kDa and 65 kDa on non-denaturing SDS-PAGE. Radioactive signals from both bands were lost by reducing the sample with β-mercaptoethanol prior to electrophoresis, in which case a radioactive fragment of <5 kDa migrated with the dye front. These results suggest that the binding of ANP to both high and low molecular weight "receptor" proteins may be associated with the hydrolysis of the peptide.

Original languageEnglish (US)
Pages (from-to)1361-1368
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume167
Issue number3
DOIs
StatePublished - Mar 30 1990
Externally publishedYes

Fingerprint

Atrial Natriuretic Factor Receptors
Sarcolemma
Atrial Natriuretic Factor
Native Polyacrylamide Gel Electrophoresis
Mercaptoethanol
Guanylate Cyclase
Electrophoresis
Crosslinking
Polyacrylamide Gel Electrophoresis
Hydrolysis
Coloring Agents
Molecular Weight
Chemical activation
Molecular weight
Binding Sites
Membranes
Peptides

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Characterisation of atrial natriuretic peptide receptors in bovine ventricular sarcolemma. / McCartney, Shirley; Aiton, James F.; Cramb, Gordon.

In: Biochemical and Biophysical Research Communications, Vol. 167, No. 3, 30.03.1990, p. 1361-1368.

Research output: Contribution to journalArticle

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