cGMP-gated conductance in retinal bipolar cells is suppressed by the photoreceptor transmitter

Scott Nawy; Craig E. Jahr

doi:10.1016/0896-6273(91)90380-I

cGMP-gated conductance in retinal bipolar cells is suppressed by the photoreceptor transmitter

Scott Nawy, Craig E. Jahr

Vollum Institute

Research output: Contribution to journal › Article › peer-review

186 Scopus citations

Abstract

Transmitter release from photoreceptors is decreased by light, resulting in a conductance increase in depolarizing bipolar cells. Addition of exogenous cGMP through a patch pipette to depolarizing bipolar cells from slices of dark-adapted tiger salamander retina resulted in an enhancement of the light response. This enhancement was blocked by GTP-γ-S and dipyridamole, an inhibitor of phosphodiesterase. GTP-γ-S and dipyridamole also blocked responses to exogenously applied 2-amino-4-phosphonobutyrate (APB), the glutamate agonist selective for this receptor. These data support the hypothesis that the postsynaptic receptor is linked via a G protein to a phosphodiesterase. The binding of glutamate or APB to the receptor suppresses a cGMP-activated current by increasing the rate of cyclic nucleotide hydrolysis.

Original language	English (US)
Pages (from-to)	677-683
Number of pages	7
Journal	Neuron
Volume	7
Issue number	4
DOIs	https://doi.org/10.1016/0896-6273(91)90380-I
State	Published - Oct 1991

ASJC Scopus subject areas

General Neuroscience

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10.1016/0896-6273(91)90380-I

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title = "cGMP-gated conductance in retinal bipolar cells is suppressed by the photoreceptor transmitter",

abstract = "Transmitter release from photoreceptors is decreased by light, resulting in a conductance increase in depolarizing bipolar cells. Addition of exogenous cGMP through a patch pipette to depolarizing bipolar cells from slices of dark-adapted tiger salamander retina resulted in an enhancement of the light response. This enhancement was blocked by GTP-γ-S and dipyridamole, an inhibitor of phosphodiesterase. GTP-γ-S and dipyridamole also blocked responses to exogenously applied 2-amino-4-phosphonobutyrate (APB), the glutamate agonist selective for this receptor. These data support the hypothesis that the postsynaptic receptor is linked via a G protein to a phosphodiesterase. The binding of glutamate or APB to the receptor suppresses a cGMP-activated current by increasing the rate of cyclic nucleotide hydrolysis.",

author = "Scott Nawy and Jahr, {Craig E.}",

note = "Funding Information: This work was supported by National Institutes of Health grants EY09139 (C. E. j.) and N. R. S. A. EY06136 (S. N.) and by the McKnight Endowment Fund for Neuroscience (C. E. J.).",

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T1 - cGMP-gated conductance in retinal bipolar cells is suppressed by the photoreceptor transmitter

AU - Nawy, Scott

AU - Jahr, Craig E.

N1 - Funding Information: This work was supported by National Institutes of Health grants EY09139 (C. E. j.) and N. R. S. A. EY06136 (S. N.) and by the McKnight Endowment Fund for Neuroscience (C. E. J.).

PY - 1991/10

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N2 - Transmitter release from photoreceptors is decreased by light, resulting in a conductance increase in depolarizing bipolar cells. Addition of exogenous cGMP through a patch pipette to depolarizing bipolar cells from slices of dark-adapted tiger salamander retina resulted in an enhancement of the light response. This enhancement was blocked by GTP-γ-S and dipyridamole, an inhibitor of phosphodiesterase. GTP-γ-S and dipyridamole also blocked responses to exogenously applied 2-amino-4-phosphonobutyrate (APB), the glutamate agonist selective for this receptor. These data support the hypothesis that the postsynaptic receptor is linked via a G protein to a phosphodiesterase. The binding of glutamate or APB to the receptor suppresses a cGMP-activated current by increasing the rate of cyclic nucleotide hydrolysis.

AB - Transmitter release from photoreceptors is decreased by light, resulting in a conductance increase in depolarizing bipolar cells. Addition of exogenous cGMP through a patch pipette to depolarizing bipolar cells from slices of dark-adapted tiger salamander retina resulted in an enhancement of the light response. This enhancement was blocked by GTP-γ-S and dipyridamole, an inhibitor of phosphodiesterase. GTP-γ-S and dipyridamole also blocked responses to exogenously applied 2-amino-4-phosphonobutyrate (APB), the glutamate agonist selective for this receptor. These data support the hypothesis that the postsynaptic receptor is linked via a G protein to a phosphodiesterase. The binding of glutamate or APB to the receptor suppresses a cGMP-activated current by increasing the rate of cyclic nucleotide hydrolysis.

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cGMP-gated conductance in retinal bipolar cells is suppressed by the photoreceptor transmitter

Abstract

ASJC Scopus subject areas

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