Central urocortin 3 administration decreases limited-access ethanol intake in nondependent mice

Amanda L. Sharpe, Tamara Phillips

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Stress and alcohol abuse are co-related. Acute alcohol is anxiolytic and stress is cited as a factor in relapse to alcohol use. A primary mediator of the stress response is the neuropeptide corticotropin-releasing factor (CRF). The CRF family of endogenous ligands includes urocortin 3 (Ucn 3), which binds selectively to the CRF type 2 receptor and has been implicated in ethanol consumption in dependent and withdrawing rats. The objective of this study was to examine the effect of Ucn 3, delivered centrally to nondependent mice, on limited-access ethanol consumption. Adult C57BL/6J mice were trained to self-administer 10% ethanol during daily, 2-h limited-access sessions, using lickometers to assess drinking patterns for both ethanol and water. Sterile saline or 0.3, 1, or 3-nmol of Ucn 3 was microinjected into the lateral ventricle immediately before the limited-access session in a within-subjects design. There was a significant decrease in ethanol (both ml and g/kg), but not water, intake following Ucn 3 treatment, explained by a change in size of the largest lick run. Food intake at both 2-h and 24-h after injection was statistically unaffected by Ucn 3 administration. These results establish a role for CRF type 2 receptors in a nondependent mouse model of ethanol self-administration.

Original languageEnglish (US)
Pages (from-to)346-351
Number of pages6
JournalBehavioural Pharmacology
Volume20
Issue number4
DOIs
StatePublished - Jul 2009

Fingerprint

Urocortins
Ethanol
Corticotropin-Releasing Hormone
Drinking
Alcohols
Self Administration
Lateral Ventricles
Anti-Anxiety Agents
Neuropeptides
Inbred C57BL Mouse
Alcoholism
Eating
Ligands
Recurrence
Injections
Water

Keywords

  • Alcohol drinking
  • Corticotrophin-releasing factor2
  • Ingestion
  • Microinjections
  • Mouse
  • Urocortin 3

ASJC Scopus subject areas

  • Pharmacology
  • Psychiatry and Mental health

Cite this

Central urocortin 3 administration decreases limited-access ethanol intake in nondependent mice. / Sharpe, Amanda L.; Phillips, Tamara.

In: Behavioural Pharmacology, Vol. 20, No. 4, 07.2009, p. 346-351.

Research output: Contribution to journalArticle

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