Central urocortin 3 administration decreases limited-access ethanol intake in nondependent mice

Amanda L. Sharpe, Tamara J. Phillips

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Stress and alcohol abuse are co-related. Acute alcohol is anxiolytic and stress is cited as a factor in relapse to alcohol use. A primary mediator of the stress response is the neuropeptide corticotropin-releasing factor (CRF). The CRF family of endogenous ligands includes urocortin 3 (Ucn 3), which binds selectively to the CRF type 2 receptor and has been implicated in ethanol consumption in dependent and withdrawing rats. The objective of this study was to examine the effect of Ucn 3, delivered centrally to nondependent mice, on limited-access ethanol consumption. Adult C57BL/6J mice were trained to self-administer 10% ethanol during daily, 2-h limited-access sessions, using lickometers to assess drinking patterns for both ethanol and water. Sterile saline or 0.3, 1, or 3-nmol of Ucn 3 was microinjected into the lateral ventricle immediately before the limited-access session in a within-subjects design. There was a significant decrease in ethanol (both ml and g/kg), but not water, intake following Ucn 3 treatment, explained by a change in size of the largest lick run. Food intake at both 2-h and 24-h after injection was statistically unaffected by Ucn 3 administration. These results establish a role for CRF type 2 receptors in a nondependent mouse model of ethanol self-administration.

Original languageEnglish (US)
Pages (from-to)346-351
Number of pages6
JournalBehavioural Pharmacology
Volume20
Issue number4
DOIs
StatePublished - Jul 1 2009

Keywords

  • Alcohol drinking
  • Corticotrophin-releasing factor2
  • Ingestion
  • Microinjections
  • Mouse
  • Urocortin 3

ASJC Scopus subject areas

  • Pharmacology
  • Psychiatry and Mental health

Fingerprint Dive into the research topics of 'Central urocortin 3 administration decreases limited-access ethanol intake in nondependent mice'. Together they form a unique fingerprint.

  • Cite this