TY - JOUR
T1 - Cellular transcripts encoded at a locus which permits retrovirus expression in mouse embryonic cells
AU - Petersen, Richard
AU - Sobel, Suzanne
AU - Wang, Chin tien
AU - Jaenisch, Rudolf
AU - Barklis, Eric
N1 - Funding Information:
We are grateful to Jeff Comer and Ivan Peckman for advice and assistance. We also acknowledge the kind gifts F9 and 14-day mouse embryo cDNA libraries from Drs. Wee-Sup Shin and Alex Stacey. Thanks also to Dr. Philip Soriano and Dr. Miles Wilkinson for DNA and RNA blots. This work was supported by grant MV416A from the American Cancer Society to E-B. Sequenced ata described in this paper have been submitted to the EM~~~~~n~ank Data libraries under accession No. YOO225.
PY - 1991/5/30
Y1 - 1991/5/30
N2 - Three independent recombinant retroviruses have been activated on insertion into the F2 locus of mouse F9 embryonal carcinoma cells. Each provirus has integrated downstream from the cellular F2 promoter, which is active in transient transfection assays using a chloramphenicol acetyltransferase reporter enzyme. The F2 promoter drives expression of a series of related transcripts in F9 and 3T3 cells, and a single 450-nt transcript in mouse tissues. F2 homologous sequences have been detected in the genomes of all mammalian species tested, and the 450-nucleotide (nt) F2 transcript is expressed in rat and human cells. Three pairs of differently sized F2 cDNA clones have been isolated and analyzed. The largest clones possess two 199-nt 98.5% identical repeats, one of which is present in the smaller clones, as well as the major 450-nt transcript. Activated proviral integration sites map to introns of the largest F2 cDNA clone. While none of the F2 cDNA contains a long open reading frame or homology to databank sequences, evidence suggests that the F2 locus encodes a constitutive function required at high levels, or represents an expressed but nonfunctional, single-copy element, conserved among mammals.
AB - Three independent recombinant retroviruses have been activated on insertion into the F2 locus of mouse F9 embryonal carcinoma cells. Each provirus has integrated downstream from the cellular F2 promoter, which is active in transient transfection assays using a chloramphenicol acetyltransferase reporter enzyme. The F2 promoter drives expression of a series of related transcripts in F9 and 3T3 cells, and a single 450-nt transcript in mouse tissues. F2 homologous sequences have been detected in the genomes of all mammalian species tested, and the 450-nucleotide (nt) F2 transcript is expressed in rat and human cells. Three pairs of differently sized F2 cDNA clones have been isolated and analyzed. The largest clones possess two 199-nt 98.5% identical repeats, one of which is present in the smaller clones, as well as the major 450-nt transcript. Activated proviral integration sites map to introns of the largest F2 cDNA clone. While none of the F2 cDNA contains a long open reading frame or homology to databank sequences, evidence suggests that the F2 locus encodes a constitutive function required at high levels, or represents an expressed but nonfunctional, single-copy element, conserved among mammals.
KW - F2 promoter
KW - Recombinant DNA provirus
KW - constitutive gene
KW - mammalian expression vectors
KW - splicing
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U2 - 10.1016/0378-1119(91)90409-5
DO - 10.1016/0378-1119(91)90409-5
M3 - Article
C2 - 1711497
AN - SCOPUS:0025860187
SN - 0378-1119
VL - 101
SP - 177
EP - 183
JO - Gene
JF - Gene
IS - 2
ER -