Cellular analysis based on moving infrared optical gradient fields

Philippe J. Marchand, Mark Wang, Catherine Schnabel, Mirianas Chachisvillis, Haichuan Zhang, Rong Yang, Laura McMullin, Norbert Hagen, Osman Kibar, Sadik Esener, Gene Tu, Sudipto Sur, Luis Pestana, Kirk Haden, Khai Truong, Wilson Seto, Kris Lykstad, Laura Simons, Michael Paliotti, Hona KarivJeffrey Hall, William Butler

Research output: Contribution to journalConference articlepeer-review

Abstract

A novel, non-invasive measurement technique for quantitative cellular analysis is presented that utilizes the forces generated by an optical beam to evaluate the physical properties of live cells in suspension. Analysis is performed by rapidly scanning a focused, near-infrared laser line with a high cross-sectional intensity gradient across a field of cells and monitoring their interaction with the beam. The response of each cell to the laser depends on its size, structure, morphology, composition, and surface membrane properties; therefore, using this technique, cell populations of different type, treatment, or biological state can be compared. To demonstrate the utility of this cell analysis platform we have evaluated the early stages of apoptosis induced in the U937 cancer cell line by the drug camptothecin and compared the results to established references assays. Measurements on our platform show detection of cellular changes earlier than either of the fluorescence-based annexin V or caspase assays. Because no labeling or additional cell processing is required and because accurate assays can be performed with a small number of cells, this measurement technique may find suitable applications in cell research, medical diagnostics, and drug discovery.

Original languageEnglish (US)
Pages (from-to)42-50
Number of pages9
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume5181
DOIs
StatePublished - Dec 1 2003
Externally publishedYes
EventWave Optics and Photonic Devices for Optical Information Processing II - San Diego, CA, United States
Duration: Aug 7 2003Aug 8 2003

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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