Cell type-specific expression of fasciclin II isoforms reveals neuronal-glial interactions during peripheral nerve growth

Jay W. Wright, Philip Copenhaver

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

During the formation of the insect peripheral nervous system (PNS), the cell adhesion receptor fasciclin II has been shown to play a prominent role in axonal fasciculation and synapse formation during motor neuron outgrowth. In the moth Manduca, fasciclin II (MFas II) is expressed both as a transmembrane isoform (TM-MFas II) and a glycosyl phosphatidylinositol-linked isoform (GPI-MFas II). By using RNA and antibody probes, we have shown that these two isoforms are expressed in nonoverlapping patterns: TM-MFas II is expressed exclusively by neurons and becomes localized to their most motile regions, while GPI-MFas II is expressed primarily by the glial cells that ensheath the peripheral nerves. This cell-type specificity of expression allowed us to monitor the nature of neuronal-glial interactions during PNS development. The outgrowth of TM-MFas II-positive axons in many regions preceded the arrival of GPI-MFas II-expressing glial processes that enwrapped them. In a few key locations, however, GPI-MFas II-positive glial cells differentiated before the arrival of the first axons and prefigured their subsequent trajectories. Prior inhibition of GPI-MFas II expression disrupted the subsequent outgrowth of axons at these locations but not elsewhere in the PNS. Our results suggest that the two isoforms of MFas II play distinct roles with respect to cellular motility and nerve formation.

Original languageEnglish (US)
Pages (from-to)24-41
Number of pages18
JournalDevelopmental Biology
Volume234
Issue number1
DOIs
StatePublished - Jun 1 2001

Fingerprint

Manduca
Peripheral Nerves
Neuroglia
Protein Isoforms
Growth
Peripheral Nervous System
Axons
fasciclin II
RNA Probes
Glycosylphosphatidylinositols
Moths
Motor Neurons
Cell Adhesion
Synapses
Insects

Keywords

  • Adhesion
  • Fasciculation
  • Growth cone
  • Insect
  • Manduca sexta

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Cell type-specific expression of fasciclin II isoforms reveals neuronal-glial interactions during peripheral nerve growth. / Wright, Jay W.; Copenhaver, Philip.

In: Developmental Biology, Vol. 234, No. 1, 01.06.2001, p. 24-41.

Research output: Contribution to journalArticle

@article{e9a37020bc0f4fdea237f592341ab25d,
title = "Cell type-specific expression of fasciclin II isoforms reveals neuronal-glial interactions during peripheral nerve growth",
abstract = "During the formation of the insect peripheral nervous system (PNS), the cell adhesion receptor fasciclin II has been shown to play a prominent role in axonal fasciculation and synapse formation during motor neuron outgrowth. In the moth Manduca, fasciclin II (MFas II) is expressed both as a transmembrane isoform (TM-MFas II) and a glycosyl phosphatidylinositol-linked isoform (GPI-MFas II). By using RNA and antibody probes, we have shown that these two isoforms are expressed in nonoverlapping patterns: TM-MFas II is expressed exclusively by neurons and becomes localized to their most motile regions, while GPI-MFas II is expressed primarily by the glial cells that ensheath the peripheral nerves. This cell-type specificity of expression allowed us to monitor the nature of neuronal-glial interactions during PNS development. The outgrowth of TM-MFas II-positive axons in many regions preceded the arrival of GPI-MFas II-expressing glial processes that enwrapped them. In a few key locations, however, GPI-MFas II-positive glial cells differentiated before the arrival of the first axons and prefigured their subsequent trajectories. Prior inhibition of GPI-MFas II expression disrupted the subsequent outgrowth of axons at these locations but not elsewhere in the PNS. Our results suggest that the two isoforms of MFas II play distinct roles with respect to cellular motility and nerve formation.",
keywords = "Adhesion, Fasciculation, Growth cone, Insect, Manduca sexta",
author = "Wright, {Jay W.} and Philip Copenhaver",
year = "2001",
month = "6",
day = "1",
doi = "10.1006/dbio.2001.0247",
language = "English (US)",
volume = "234",
pages = "24--41",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Cell type-specific expression of fasciclin II isoforms reveals neuronal-glial interactions during peripheral nerve growth

AU - Wright, Jay W.

AU - Copenhaver, Philip

PY - 2001/6/1

Y1 - 2001/6/1

N2 - During the formation of the insect peripheral nervous system (PNS), the cell adhesion receptor fasciclin II has been shown to play a prominent role in axonal fasciculation and synapse formation during motor neuron outgrowth. In the moth Manduca, fasciclin II (MFas II) is expressed both as a transmembrane isoform (TM-MFas II) and a glycosyl phosphatidylinositol-linked isoform (GPI-MFas II). By using RNA and antibody probes, we have shown that these two isoforms are expressed in nonoverlapping patterns: TM-MFas II is expressed exclusively by neurons and becomes localized to their most motile regions, while GPI-MFas II is expressed primarily by the glial cells that ensheath the peripheral nerves. This cell-type specificity of expression allowed us to monitor the nature of neuronal-glial interactions during PNS development. The outgrowth of TM-MFas II-positive axons in many regions preceded the arrival of GPI-MFas II-expressing glial processes that enwrapped them. In a few key locations, however, GPI-MFas II-positive glial cells differentiated before the arrival of the first axons and prefigured their subsequent trajectories. Prior inhibition of GPI-MFas II expression disrupted the subsequent outgrowth of axons at these locations but not elsewhere in the PNS. Our results suggest that the two isoforms of MFas II play distinct roles with respect to cellular motility and nerve formation.

AB - During the formation of the insect peripheral nervous system (PNS), the cell adhesion receptor fasciclin II has been shown to play a prominent role in axonal fasciculation and synapse formation during motor neuron outgrowth. In the moth Manduca, fasciclin II (MFas II) is expressed both as a transmembrane isoform (TM-MFas II) and a glycosyl phosphatidylinositol-linked isoform (GPI-MFas II). By using RNA and antibody probes, we have shown that these two isoforms are expressed in nonoverlapping patterns: TM-MFas II is expressed exclusively by neurons and becomes localized to their most motile regions, while GPI-MFas II is expressed primarily by the glial cells that ensheath the peripheral nerves. This cell-type specificity of expression allowed us to monitor the nature of neuronal-glial interactions during PNS development. The outgrowth of TM-MFas II-positive axons in many regions preceded the arrival of GPI-MFas II-expressing glial processes that enwrapped them. In a few key locations, however, GPI-MFas II-positive glial cells differentiated before the arrival of the first axons and prefigured their subsequent trajectories. Prior inhibition of GPI-MFas II expression disrupted the subsequent outgrowth of axons at these locations but not elsewhere in the PNS. Our results suggest that the two isoforms of MFas II play distinct roles with respect to cellular motility and nerve formation.

KW - Adhesion

KW - Fasciculation

KW - Growth cone

KW - Insect

KW - Manduca sexta

UR - http://www.scopus.com/inward/record.url?scp=0035371363&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035371363&partnerID=8YFLogxK

U2 - 10.1006/dbio.2001.0247

DO - 10.1006/dbio.2001.0247

M3 - Article

VL - 234

SP - 24

EP - 41

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 1

ER -